ArticleMolecular biology of the cell2007
A role for clathrin in reassembly of the Golgi apparatus.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- CLCa mediates a novel cross-talk between Wnt secretion and actin organization.Life science alliance · 2025Article
- A non-canonical role of ATG8 in Golgi recovery from heat stress in plants.Nature plants · 2023Article
- Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada.BMC biology · 2022Article
- Clathrin packets move in slow axonal transport and deliver functional payloads to synapses.Neuron · 2021Article
- Clathrin Heavy Chain 1 Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep.Frontiers in cell and developmental biology · 2021Article
- Role of clathrin-mediated endocytosis in the use of heme and hemoglobin by the fungal pathogen Cryptococcus neoformans.Cellular microbiology · 2019Article
- Ubiquitination mediates Kv1.3 endocytosis as a mechanism for protein kinase C-dependent modulation.Scientific reports · 2017Article
- Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice.Biochemistry research international · 2016Article
- Synthesis of the Pitstop family of clathrin inhibitors.Nature protocols · 2014Article
- Clathrin-coated vesicles from brain have small payloads: a cryo-electron tomographic study.Journal of structural biology · 2013Article
- Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells.Molecular cancer · 2013Article
- Clathrin is required for postmitotic Golgi reassembly.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- The Golgi protein p115 associates with gamma-tubulin and plays a role in Golgi structure and mitosis progression.The Journal of biological chemistry · 2011Article
- Respective implications of glutamate decarboxylase antibodies in stiff person syndrome and cerebellar ataxia.Orphanet journal of rare diseases · 2011Article
- Fragmentation of the Golgi apparatus provides replication membranes for human rhinovirus 1A.Virology · 2010Article
- Vesicle formation and endocytosis: function, machinery, mechanisms, and modeling.Antioxidants & redox signaling · 2009Review
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Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The Golgi apparatus is a highly dynamic organelle whose organization is maintained by a proteinaceous matrix, cytoskeletal components, and inositol phospholipids. In mammalian cells, disassembly of the organelle occurs reversibly at the onset of mitosis and irreversibly during apoptosis. Several pharmacological agents including nocodazole, brefeldin A (BFA), and primary alcohols (1-butanol) induce reversible fragmentation of the Golgi apparatus. To dissect the mechanism of Golgi reassembly, rat NRK and GH3 cells were treated with 1-butanol, BFA, or nocodazole. During washout of 1-butanol, clathrin, a ubiquitous coat protein implicated in vesicle traffic at the trans-Golgi network and plasma membrane, and abundant clathrin coated vesicles were recruited to the region of nascent Golgi cisternae. Knockdown of endogenous clathrin heavy chain showed that the Golgi apparatus failed to reform efficiently after BFA or 1-butanol removal. Instead, upon 1-butanol washout, it maintained a compact, tight morphology. Our results suggest that clathrin is required to reassemble fragmented Golgi elements. In addition, we show that after butanol treatment the Golgi apparatus reforms via an initial compact intermediate structure that is subsequently remodeled into the characteristic interphase lace-like morphology and that reassembly requires clathrin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.