ArticleThe Journal of molecular diagnostics : JMD2006
A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogene.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- The bacterial microbiota in first-void urine from men with and without idiopathic urethritis.PloS one · 2018Trial
- Development of a Point-of-Care Recombinase Polymerase Amplification Assay for Detection of Neisseria gonorrhoeae and Ceftriaxone-Resistance Associated penA Allele 60.001.Molecular diagnosis & therapy · 2026Article
- Development and Laboratory Evaluation of aInternational journal of molecular sciences · 2026Article
- Whole-genome characterization of seven multidrug-resistantFrontiers in microbiology · 2026Article
- A low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.The Analyst · 2025Article
- Clinical Validation of Loop-Mediated Isothermal Amplification Assays for the Rapid Detection of Neisseria gonorrhoeae and Chlamydia trachomatis.Microbial biotechnology · 2025Article
- An updated molecular diagnostic for surveillance ofmedRxiv : the preprint server for health sciences · 2024Article
- Promoting molecular diagnostic equity: assessing in-house real-time PCR forLe infezioni in medicina · 2024Article
- Article
- Co-infection of human papillomavirus and other sexually transmitted bacteria in cervical cancer patients in the Philippines.Gynecologic oncology reports · 2022Article
- High-Resolution Melting Analysis to Detect Antimicrobial Resistance Determinants in South AfricanInternational journal of microbiology · 2022Article
- Genomic evolution of Neisseria gonorrhoeae since the preantibiotic era (1928-2013): antimicrobial use/misuse selects for resistance and drives evolution.BMC genomics · 2020Article
- Prevalence and factors associated with gonorrhea infection with respect to anatomic distributions among men who have sex with men.PloS one · 2019Article
- Multidrug-resistantEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2018Article
- Prevalence of Sexually Transmitted Infections among Married Women in Rural Nepal.Infectious diseases in obstetrics and gynecology · 2018Article
- Loop-mediated isothermal amplification of Neisseria gonorrhoeae porA pseudogene: a rapid and reliable method to detect gonorrhea.AMB Express · 2017Article
- Sexually Transmitted Infections: A Novel Screening Strategy for Improving Women's Health in Vulnerable Populations.International journal of molecular sciences · 2017Article
- Influence of storage time on DNA of Chlamydia trachomatis, Ureaplasma urealyticum, and Neisseria gonorrhoeae for accurate detection by quantitative real-time polymerase chain reaction.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2016Article
- New Clinical Strain of Neisseria gonorrhoeae with Decreased Susceptibility to Ceftriaxone, Japan.Emerging infectious diseases · 2016Article
- Mycoplasma genitalium infection is associated with microscopic signs of cervical inflammation in liquid cytology specimens.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ever since the advent of molecular methods, the diagnostics of Neisseria gonorrhoeae has been troubled by false negative and false positive results compared with culture. Commensal Neisseria species and Neisseria meningitidis are closely related to N. gonorrhoeae and may cross-react when using molecular tests comprising too-low specificity. We have devised a real-time polymerase chain reaction (PCR), including an internal amplification control, that targets the N. gonorrhoeae porA pseudogene. DNA was automatically isolated on a BioRobot M48. Our subsequent PCR method amplified all of the different N. gonorrhoeae international reference strains (n = 34) and N. gonorrhoeae clinical isolates (n = 176) but not isolates of the 13 different nongonococcal Neisseria species (n = 68) that we tested. Furthermore, a panel of gram-negative bacterial (n = 18), gram-positive bacterial (n = 23), fungal (n = 1), and viral (n = 4) as well as human DNA did not amplify. The limit of detection was determined to be less than 7.5 genome equivalents/PCR reaction. In conclusion, the N. gonorrhoeae porA pseudogene real-time PCR developed in the present study is highly sensitive, specific, robust, rapid and reproducible, making it suitable for diagnosis of N. gonorrhoeae infection.
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