ArticleJournal of clinical microbiology2006
Use of internally controlled real-time genome amplification for detection of variola virus and other orthopoxviruses infecting humans.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Beyond pathogen-specific preparedness: adaptable laboratory systems for emerging infections.The Lancet regional health. Europe · 2026Article
- Changes in HIV-1 Reservoir Dynamics After Mpox Infection.Journal of medical virology · 2025Article
- A Fatal Case of Monkeypox-Associated Encephalitis in a Non-HIV-Infected Patient.Critical care explorations · 2025Article
- Development of a new quantitative RT-PCR to detect lymphocytic choriomeningitis virus.Frontiers in veterinary science · 2025Article
- Monkeypox virus genomic accordion strategies.Nature communications · 2024Article
- Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain.Emerging infectious diseases · 2023Article
- Internal Validation of a Real-Time qPCR Kit following the UNE/EN ISO/IEC 17025:2005 for Detection of the Re-EmergingDiagnostics (Basel, Switzerland) · 2023Article
- Review
- Monkeypox outbreak in Madrid (Spain): Clinical and virological aspects.The Journal of infection · 2022Observational
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- Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay.Journal of virological methods · 2011Article
- Development of real-time PCR assay for specific detection of cowpox virus.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2010Article
- Orthopoxvirus detection in environmental specimens during suspected bioterror attacks: inhibitory influences of common household products.Applied and environmental microbiology · 2008Article
- Synergistic efficacy of the combination of ST-246 with CMX001 against orthopoxviruses.Antimicrobial agents and chemotherapy · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Smallpox, once a devastating disease caused by Variola virus, a member of the Orthopoxvirus genus, was eradicated in 1980. However, the importance of variola virus infections has been stressed widely in the last few years, particularly following recent social events in the world. Today, variola virus is considered to be one of the most significant agents with potential use as a biological weapon. In this study we developed an internally controlled real-time PCR assay for rapid detection and simultaneous differentiation of variola virus from other orthopoxviruses. The assay is based on TaqMan 3'-minor groove binder (MGB) chemistry and uses generic primers, designed in highly conserved genomic regions of the crmB gene, and three TaqMan MGB probes designed to identify orthopoxviruses, variola virus, and an internal control. The results obtained suggest that the assay is rapid, sensitive, specific, and suitable for the generic detection of orthopoxviruses and the identification of variola virus and avoids false-negative results in a single reaction tube.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.