ArticleAmerican journal of physiology. Cell physiology2007
Ca2+ sparks and T tubule reorganization in dedifferentiating adult mouse skeletal muscle fibers.
Article in American journal of physiology. Cell physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 42 citations in OpenAlex.
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- Effects of myofiber isolation technique on sarcolemma biomechanics.BioTechniques · 2020Article
- A Strategy for Discovery of Endocrine Interactions with Application to Whole-Body Metabolism.Cell metabolism · 2018Article
- Characterization and utilization of the flexor digitorum brevis for assessing skeletal muscle function.Skeletal muscle · 2018Article
- Calcium-induced release of calcium in muscle: 50 years of work and the emerging consensus.The Journal of general physiology · 2018Review
- Acute Elevated Glucose Promotes Abnormal Action Potential-Induced CaJournal of diabetes research · 2017Article
- Alternating bipolar field stimulation identifies muscle fibers with defective excitability but maintained local Ca(2+) signals and contraction.Skeletal muscle · 2016Article
- Disruption of action potential and calcium signaling properties in malformed myofibers from dystrophin-deficient mice.Physiological reports · 2015Article
- Dysferlin stabilizes stress-induced Ca2+ signaling in the transverse tubule membrane.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Elevated extracellular glucose and uncontrolled type 1 diabetes enhance NFAT5 signaling and disrupt the transverse tubular network in mouse skeletal muscle.Experimental biology and medicine (Maywood, N.J.) · 2012Article
- Triclosan impairs excitation-contraction coupling and Ca2+ dynamics in striated muscle.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- NOX2-dependent ROS is required for HDAC5 nuclear efflux and contributes to HDAC4 nuclear efflux during intense repetitive activity of fast skeletal muscle fibers.American journal of physiology. Cell physiology · 2012Article
- Structural and functional evaluation of branched myofibers lacking intermediate filaments.American journal of physiology. Cell physiology · 2012Article
- Measuring mitochondrial respiration in intact single muscle fibers.American journal of physiology. Regulatory, integrative and comparative physiology · 2012Article
- Genetic deletion of trkB.T1 increases neuromuscular function.American journal of physiology. Cell physiology · 2012Article
- Differential impact of mitochondrial positioning on mitochondrial Ca(2+) uptake and Ca(2+) spark suppression in skeletal muscle.American journal of physiology. Cell physiology · 2011Article
- S100A1 and calmodulin regulation of ryanodine receptor in striated muscle.Cell calcium · 2011Review
- Modulation of contractile apparatus Ca2+ sensitivity and disruption of excitation-contraction coupling by S-nitrosoglutathione in rat muscle fibres.The Journal of physiology · 2011Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
Ca(+) sparks are rare in healthy adult mammalian skeletal muscle but may appear when adult fiber integrity is compromised, and occur in embryonic muscle but decline as the animal develops. Here we used cultured adult mouse flexor digitorum brevis muscle fibers to monitor occurrence of Ca(2+) sparks during maintenance of adult fiber morphology and during eventual fiber morphological dedifferentiation after various times in culture. Fibers cultured for up to 3 days retain normal morphology and striated appearance. Ca(2+) sparks were rare in these fibers. At 5-7 days in culture, many of the original muscle fibers exhibit sprouting and loss of striations, as well as the occurrence of spontaneous Ca(2+) sparks. The average rate of occurrence of Ca(2+) sparks is >10-fold higher after 5-7 days in culture than in days 1-3. With the use of fibers cultured for 7 days, application of the Ca(2+) channel blockers Co(2+) or nifedipine almost completely suppressed the occurrence of Ca(2+) sparks, as previously shown in embryonic fibers, suggesting that Ca(2+) sparks may be generated by similar mechanisms in dedifferentiating cultured adult fibers and in embryonic fibers before final differentiation. The sarcomeric disruption observed under transmitted light microscopy in dedifferentiating fibers was accompanied by morphological changes in the transverse (T) tubular system, as observed by fluorescence confocal imaging of both an extracellular marker dye and membrane staining dyes. Changes in T tubule morphology coincided with the appearance of Ca(2+) sparks, suggesting that Ca(2+) sparks may either be a signal for, or the result of, disruption of DHPR-ryanodine receptor 1 coupling.
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