ArticleProceedings of the National Academy of Sciences of the United States of America2006
Independent and simultaneous translocation of two substrates by a nucleotide sugar transporter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Delivery of Nucleotide Sugars to the Mammalian Golgi: A Very Well (un)Explained Story.International journal of molecular sciences · 2022Review
- Gateway to the Golgi: molecular mechanisms of nucleotide sugar transporters.Current opinion in structural biology · 2019Review
- Conserved Glu-47 and Lys-50 residues are critical for UDP-The Journal of biological chemistry · 2019Article
- Structural basis for the delivery of activated sialic acid into Golgi for sialyation.Nature structural & molecular biology · 2019Article
- My journey in the discovery of nucleotide sugar transporters of the Golgi apparatus.The Journal of biological chemistry · 2018Article
- Article
- Identification of a Golgi-localized UDP-N-acetylglucosamine transporter in Trypanosoma cruzi.BMC microbiology · 2015Article
- A nucleotide sugar transporter involved in glycosylation of the Toxoplasma tissue cyst wall is required for efficient persistence of bradyzoites.PLoS pathogens · 2013Article
- Developmental diseases caused by impaired nucleotide sugar transporters.Glycoconjugate journal · 2013Review
- Heparan sulfate biosynthesis: regulation and variability.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Review
- Comparative analysis of involvement of UGT1 and UGT2 splice variants of UDP-galactose transporter in glycosylation of macromolecules in MDCK and CHO cell lines.Glycoconjugate journal · 2011Article
- Article
- The role of nucleotide sugar transporters in development of eukaryotes.Seminars in cell & developmental biology · 2010Review
- Cosmc is an essential chaperone for correct protein O-glycosylation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The ortholog of human solute carrier family 35 member B1 (UDP-galactose transporter-related protein 1) is involved in maintenance of ER homeostasis and essential for larval development in Caenorhabditis elegans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Two novel nucleobase/pentamidine transporters from Trypanosoma brucei.Molecular and biochemical parasitology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nucleotide sugar transporters play an essential role in protein and lipid glycosylation, and mutations can result in developmental phenotypes. We have characterized a transporter of UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine encoded by the Caenorhabditis elegans gene C03H5.2. Surprisingly, translocation of these substrates occurs in an independent and simultaneous manner that is neither a competitive nor a symport transport. Incubations of Golgi apparatus vesicles of Saccharomyces cerevisiae expressing C03H5.2 protein with these nucleotide sugars labeled with (3)H and (14)C in their sugars showed that both substrates enter the lumen to the same extent, whether or not they are incubated alone or in the presence of a 10-fold excess of the other nucleotide sugar. Vesicles containing a deletion mutant of the C03H5.2 protein transport UDP-N-acetylglucosamine at rates comparable with that of wild-type transporter, whereas transport of UDP-N-acetylgalactosamine was decreased by 85-90%, resulting in an asymmetrical loss of substrate transport.
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Registered trials
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