ArticlePlant physiology2006
Localization and role of manganese superoxide dismutase in a marine diatom.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 94 citations in OpenAlex.
- The cryo-EM structure of Photosystem I from Chromera velia with a bound superoxide dismutase heterodimer.Nature communications · 2025Article
- LASSO Regression with Multiple Imputations for the Selection of Key Variables Affecting the Fatty Acid Profile ofMarine drugs · 2023Article
- Spatial Heterogeneity in Particle-Associated, Light-Independent Superoxide Production Within Productive Coastal Waters.Journal of geophysical research. Oceans · 2020Article
- Insights on the Functions and Ecophysiological Relevance of the Diverse Carbonic Anhydrases in Microalgae.International journal of molecular sciences · 2020Review
- Insights into the respiratory chain and oxidative stress.Bioscience reports · 2018Review
- Physicochemical characteristics and toxicity of surface-modified zinc oxide nanoparticles to freshwater and marine microalgae.Scientific reports · 2017Article
- Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome.eLife · 2017Article
- Iron mediates catalysis of nucleic acid processing enzymes: support for Fe(II) as a cofactor before the great oxidation event.Nucleic acids research · 2017Article
- Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice.Plant biotechnology journal · 2015Article
- Article expression, purification, and characterization of Cu/ZnSOD from Panax ginseng.Molecules (Basel, Switzerland) · 2014Article
- Manganese the protector.Nature chemistry · 2013Article
- RNA with iron(II) as a cofactor catalyses electron transfer.Nature chemistry · 2013Article
- The ins and outs of algal metal transport.Biochimica et biophysica acta · 2012Review
- Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii.The Plant cell · 2012Article
- Phytoplankton responses to atmospheric metal deposition in the coastal and open-ocean Sargasso Sea.Frontiers in microbiology · 2012Article
- Elemental economy: microbial strategies for optimizing growth in the face of nutrient limitation.Advances in microbial physiology · 2012Review
- Feedback Interactions between Trace Metal Nutrients and Phytoplankton in the Ocean.Frontiers in microbiology · 2012Article
- RNA folding and catalysis mediated by iron (II).PloS one · 2012Article
- Oxidative stress resistance in Deinococcus radiodurans.Microbiology and molecular biology reviews : MMBR · 2011Review
- Auxiliary electron transport pathways in chloroplasts of microalgae.Photosynthesis research · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superoxide dismutase (SOD) catalyzes the transformation of superoxide to molecular oxygen and hydrogen peroxide. Of the four known SOD isoforms, distinguished by their metal cofactor (iron, manganese [Mn], copper/zinc, nickel), MnSOD is the dominant form in the diatom Thalassiosira pseudonana. We cloned the MnSOD gene, sodA, using the expression vector pBAD, overexpressed the product in Escherichia coli, and purified the mature protein (TpMnSOD). This recombinant enzyme was used to generate a polyclonal antibody in rabbit that recognizes MnSOD in T. pseudonana. Based on quantitative immunoblots, we calculate that in vivo concentrations of TpMnSOD are approximately 0.9 amol cell(-1) using the recombinant protein as a standard. Immunogold staining indicates that TpMnSOD is localized in the chloroplasts, which is in contrast to most other eukaryotic algae (including chlorophytes and embryophytes) where MnSOD is localized exclusively in mitochondria. Based on the photosynthetic Mn complex in photosystem II, cellular Mn budgets cannot account for 50% to 80% of measured Mn within diatom cells. Our results reveal that chloroplastic MnSOD accounts for 10% to 20% of cellular Mn, depending on incident light intensity and cellular growth rate. Indeed, our analysis indicates that TpMnSOD accounts for 1.4% (+/-0.2%) of the total protein in the cell. The TpMnSOD has a rapid turnover rate with an apparent half-life of 6 to 8 h when grown under continuous light. TpMnSOD concentrations increase relative to chlorophyll, with an increase in incident light intensity to minimize photosynthetic oxidative stress. The employment of a Mn-based SOD, linked to photosynthetic stress in T. pseudonana, may contribute to the continued success of diatoms in the low iron regions of the modern ocean.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.