ArticleBiophysical journal2007
pH-Dependence of extrinsic and intrinsic H(+)-ion mobility in the rat ventricular myocyte, investigated using flash photolysis of a caged-H(+) compound.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Cell Settling, Migration, and Stochastic Cancer Gene Expression Suggest Potassium Membrane Flux May Initiate pH Reversal.Biomolecules · 2025Article
- Cardiomyocyte NaFrontiers in cardiovascular medicine · 2020Article
- Distinct moieties underlie biphasic HFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Regional acidosis locally inhibits but remotely stimulates Ca2+ waves in ventricular myocytes.Cardiovascular research · 2017Article
- Red blood cell thickness is evolutionarily constrained by slow, hemoglobin-restricted diffusion in cytoplasm.Scientific reports · 2016Article
- Stromal uptake and transmission of acid is a pathway for venting cancer cell-generated acid.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Protons Trigger Mitochondrial Flashes.Biophysical journal · 2016Article
- Nuclear proton dynamics and interactions with calcium signaling.Journal of molecular and cellular cardiology · 2016Article
- Na⁺ ions as spatial intracellular messengers for co-ordinating Ca²⁺ signals during pH heterogeneity in cardiomyocytes.Cardiovascular research · 2015Article
- Pumping Ca2+ up H+ gradients: a Ca2(+)-H+ exchanger without a membrane.The Journal of physiology · 2014Review
- Facilitation by intracellular carbonic anhydrase of Na+ -HCO3- co-transport but not Na+ / H+ exchange activity in the mammalian ventricular myocyte.The Journal of physiology · 2014Article
- Coupled Ca2+/H+ transport by cytoplasmic buffers regulates local Ca2+ and H+ ion signaling.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Sarcolemmal localisation of Na+/H+ exchange and Na+-HCO3- co-transport influences the spatial regulation of intracellular pH in rat ventricular myocytes.The Journal of physiology · 2013Article
- Effect of nitric oxide donors S-nitroso-N-acetyl-DL-penicillamine, spermine NONOate and propylamine propylamine NONOate on intracellular pH in cardiomyocytes.Clinical and experimental pharmacology & physiology · 2012Article
- Cytosolic H+ microdomain developed around AE1 during AE1-mediated Cl-/HCO3- exchange.The Journal of physiology · 2011Article
- New insights into the physiological role of carbonic anhydrase IX in tumour pH regulation.Oncogene · 2010Review
- Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle's disease.European journal of applied physiology · 2009Article
- Voltage-gated proton channels: what's next?The Journal of physiology · 2008Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Passive H(+)-ion mobility within eukaryotic cells is low, due to H(+)-ion binding to cytoplasmic buffers. A localized intracellular acidosis can therefore persist for seconds or even minutes. Because H(+)-ions modulate so many biological processes, spatial intracellular pH (pH(i))-regulation becomes important for coordinating cellular activity. We have investigated spatial pH(i)-regulation in single and paired ventricular myocytes from rat heart by inducing a localized intracellular acid-load, while confocally imaging pH(i) using the pH-fluorophore, carboxy-SNARF-1. We present a novel method for localizing the acid-load. This involves the intracellular photolytic uncaging of H(+)-ions from a membrane-permeant acid-donor, 2-nitrobenzaldehyde. The subsequent spatial pH(i)-changes are consistent with intracellular H(+)-mobility and cell-to-cell H(+)-permeability constants measured using more conventional acid-loading techniques. We use the method to investigate the effect of reducing pH(i) on intrinsic (non-CO(2)/HCO(3)(-) buffer-dependent) and extrinsic (CO(2)/HCO(3)(-) buffer-dependent) components of H(i)(+)-mobility. We find that although both components mediate spatial regulation of pH within the cell, their ability to do so declines sharply at low pH(i). Thus acidosis severely slows intracellular H(+)-ion movement. This can result in spatial pH(i) nonuniformity, particularly during the stimulation of sarcolemmal Na(+)-H(+) exchange. Intracellular acidosis thus presents a window of vulnerability in the spatial coordination of cellular function.
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