ArticleProceedings of the National Academy of Sciences of the United States of America2006
Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- Endogenous trans-translation structure visualizes the decoding of the first tmRNA alanine codon.Frontiers in microbiology · 2024Article
- Review
- Structures of tmRNA and SmpB as they transit through the ribosome.Nature communications · 2021Article
- Joachim Frank's Binding with the Ribosome.Structure (London, England : 1993) · 2019Review
- Structures of Mycobacterium smegmatis 70S ribosomes in complex with HPF, tmRNA, and P-tRNA.Scientific reports · 2018Article
- Ribosome-based quality control of mRNA and nascent peptides.Wiley interdisciplinary reviews. RNA · 2017Review
- In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution.RNA (New York, N.Y.) · 2014Article
- Requirements for resuming translation in chimeric transfer-messenger RNAs of Escherichia coli and Mycobacterium tuberculosis.BMC molecular biology · 2014Article
- An unusual mechanism for EF-Tu activation during tmRNA-mediated ribosome rescue.RNA (New York, N.Y.) · 2014Article
- The SmpB C-terminal tail helps tmRNA to recognize and enter stalled ribosomes.Frontiers in microbiology · 2014Review
- Biochemical aspects of bacterial strategies for handling the incomplete translation processes.Frontiers in microbiology · 2014Review
- tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell.Frontiers in genetics · 2014Review
- Trans-translation exposed: understanding the structures and functions of tmRNA-SmpB.Frontiers in microbiology · 2014Review
- Mechanism of trans-translation revealed by in vitro studies.Frontiers in microbiology · 2014Review
- Article
- Decoding in the absence of a codon by tmRNA and SmpB in the ribosome.Science (New York, N.Y.) · 2012Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- The role of SmpB and the ribosomal decoding center in licensing tmRNA entry into stalled ribosomes.RNA (New York, N.Y.) · 2011Article
- tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation.Journal of nucleic acids · 2011Article
- tmRNA-SmpB: a journey to the centre of the bacterial ribosome.The EMBO journal · 2010Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
In eubacterial translation, lack of a stop codon on the mRNA results in a defective, potentially toxic polypeptide stalled on the ribosome. Bacteria possess a specialized mRNA, called transfer messenger RNA (tmRNA), to rescue such a stalled system. tmRNA contains a transfer RNA (tRNA)-like domain (TLD), which enters the ribosome as a tRNA and places an ORF into the mRNA channel. This ORF codes for a signal marking the polypeptide for degradation and ends in a stop codon, leading to release of the faulty polypeptide and recycling of the ribosome. The binding of tmRNA to the stalled ribosome is mediated by small protein B (SmpB). By means of cryo-EM, we obtained a density map for the preaccommodated state of the tmRNA.SmpB.EF-Tu.70S ribosome complex with much improved definition for the tmRNA-SmpB complex, showing two SmpB molecules bound per ribosome, one toward the A site on the 30S subunit side and the other bound to the 50S subunit near the GTPase-associated center. tmRNA is strongly attached to the 30S subunit head by multiple contact sites, involving most of its pseudoknots and helices. The map clarifies that the TLD is located near helix 34 and protein S19 of the 30S subunit, rather than in the A site as tRNA for normal translation, so that the TLD is oriented toward the ORF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.