ArticleThe Plant cell2006
Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed, 196 citations in OpenAlex.
- Comparative genomics and time-course transcriptomics uncover homoeologous exchange events reshaping glucosinolate metabolism in Brassica napus.Molecular horticulture · 2026Article
- Branched-chain amino acid catabolism initiates volatile synthesis in Gentiana triflora.Planta · 2025Article
- Assembly and comparative analysis of the complete mitochondrial genome of the spice plant Cinnamomum longepaniculatum.BMC plant biology · 2025Article
- Deciphering the heterogeneous glucosinolates composition in leaves and seeds: strategies for developing Brassica napus genotypes with low seed glucosinolates content but high leaf glucosinolates content.Molecular horticulture · 2025Article
- The branched-chain amino acid-related isoleucic acid: recent research advances.Plant biology (Stuttgart, Germany) · 2025Review
- Sulforaphane in cancer precision medicine: from biosynthetic origins to multiscale mechanisms and clinical translation.Frontiers in immunology · 2025Review
- Transcriptional activation ofFrontiers in plant science · 2025Article
- Integrated Analysis of Metabolome and Transcriptome Reveals the Effect of Burdock Fructooligosaccharide on the Quality of Chinese Cabbage (International journal of molecular sciences · 2024Article
- Effect of sodium selenite on the synthesis of glucosinolates and antioxidant capacity in Chinese cabbage (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Improved pokeweed genome assembly and early gene expression changes in response to jasmonic acid.BMC plant biology · 2024Article
- Natural Products That Contain Higher Homologated Amino Acids.Chembiochem : a European journal of chemical biology · 2024Review
- Traits linked to natural variation of sulfur content in Arabidopsis thaliana.Journal of experimental botany · 2024Article
- Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review.Frontiers in plant science · 2024Review
- Evolution and comparative transcriptome analysis of glucosinolate pathway genes inFrontiers in plant science · 2024Article
- Transcriptome Dynamics ofInternational journal of molecular sciences · 2023Article
- Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways.Plant communications · 2023Review
- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.Genes · 2023Article
- Genetic Improvement ofPlants (Basel, Switzerland) · 2023Review
- Glutamic Acid and Poly-γ-glutamic Acid Enhanced the Heat Resistance of Chinese Cabbage (International journal of molecular sciences · 2022Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
As part of our analysis of branched-chain amino acid metabolism in plants, we analyzed the function of Arabidopsis thaliana BRANCHED-CHAIN AMINOTRANSFERASE4 (BCAT4). Recombinant BCAT4 showed high efficiency with Met and its derivatives and the corresponding 2-oxo acids, suggesting its participation in the chain elongation pathway of Met-derived glucosinolate biosynthesis. This was substantiated by in vivo analysis of two BCAT4 T-DNA knockout mutants, in which Met-derived aliphatic glucosinolate accumulation is reduced by approximately 50%. The increase in free Met and S-methylmethionine levels in these mutants, together with in vitro substrate specificity, strongly implicate BCAT4 in catalysis of the initial deamination of Met to 4-methylthio-2-oxobutyrate. BCAT4 transcription is induced by wounding and is predominantly observed in the phloem. BCAT4 transcript accumulation also follows a diurnal rhythm, and green fluorescent protein tagging experiments and subcellular protein fractions show that BCAT4 is located in the cytosol. The assignment of BCAT4 to the Met chain elongation pathway documents the close evolutionary relationship of this pathway to Leu biosynthesis. In addition to BCAT4, the enzyme methylthioalkylmalate synthase 1 has been recruited for the Met chain elongation pathway from a gene family involved in Leu formation. This suggests that the two pathways have a common evolutionary origin.
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