ArticleApplied and environmental microbiology2007
Identification of Escherichia coli O157:H7 genomic regions conserved in strains with a genotype associated with human infection.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- CommensalAnimals : an open access journal from MDPI · 2023Article
- Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle.PloS one · 2021Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Review
- Multilocus genotype analysis of Escherichia coli O157 isolates from Australia and the United States provides evidence of geographic divergence.Applied and environmental microbiology · 2013Article
- Study of polymorphisms in tir, eae and tccP2 genes in enterohaemorrhagic and enteropathogenic Escherichia coli of serogroup O26.BMC microbiology · 2011Article
- Genome signatures of Escherichia coli O157:H7 isolates from the bovine host reservoir.Applied and environmental microbiology · 2011Article
- Pan-genome sequence analysis using Panseq: an online tool for the rapid analysis of core and accessory genomic regions.BMC bioinformatics · 2010Article
- Lineage and host source are both correlated with levels of Shiga toxin 2 production by Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2010Article
- Escherichia coli O157:H7 strain origin, lineage, and Shiga toxin 2 expression affect colonization of cattle.Applied and environmental microbiology · 2009Article
- In silico genomic analyses reveal three distinct lineages of Escherichia coli O157:H7, one of which is associated with hyper-virulence.BMC genomics · 2009Article
- Genomic instability in regions adjacent to a highly conserved pch prophage in Escherichia coli O157:H7 generates diversity in expression patterns of the LEE pathogenicity island.Journal of bacteriology · 2009Article
- Genomic regions conserved in lineage II Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2009Article
- Super-shedding and the link between human infection and livestock carriage of Escherichia coli O157.Nature reviews. Microbiology · 2008Review
- The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates.Journal of bacteriology · 2008Article
- Genetic classification of severe early childhood caries by use of subtracted DNA fragments from Streptococcus mutans.Journal of clinical microbiology · 2008Article
- Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2008Article
- Variation in virulence among clades of Escherichia coli O157:H7 associated with disease outbreaks.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12.Journal of bacteriology · 2007Article
- Genome evolution in major Escherichia coli O157:H7 lineages.BMC genomics · 2007Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Beta-glucuronidase-negative, sorbitol-nonfermenting isolates of Shiga toxin-producing Escherichia coli O157 comprise part of a clone complex of related enterohemorrhagic E. coli isolates. High-resolution genotyping shows that the O157 populations have diverged into two different lineages that appear to have different ecologies. To identify genomic regions unique to the most common human-associated genotype, suppression subtractive hybridization was used to identify DNA sequences present in two clinical strains representing the human lineage I O157:H7 strains but absent from two bovine-derived lineage II strains. PCR assays were then used to test for the presence of these regions in 10 lineage I strains and 20 lineage II strains. Twelve conserved regions of genomic difference for lineage I (CRD(I)) were identified that were each present in at least seven of the lineage I strains but absent in most of the lineage II strains tested. The boundaries of the lineage I conserved regions were further delimited by PCR. Eleven of these CRD(I) were associated with E. coli Sakai S-loops 14, 16, 69, 72, 78, 82, 83, 91 to 93, 153, and 286, and the final CRD(I) was located on the pO157 virulence plasmid. Several potential virulence factors were identified within these regions, including a putative hemolysin-activating protein, an iron transport system, and several possible regulatory genes. Cluster analysis based on lineage I conserved regions showed that the presence/absence of these regions was congruent with the inferred phylogeny of the strains.
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