ArticleDevelopmental biology2007
Muscle development in Ciona intestinalis requires the b-HLH myogenic regulatory factor gene Ci-MRF.
Article in Developmental biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- CRISPR/Cas9 Protocols for Disrupting Gene Function in the Non-vertebrate Chordate Ciona.Integrative and comparative biology · 2024Review
- A change in cis-regulatory logic underlying obligate versus facultative muscle multinucleation in chordates.Development (Cambridge, England) · 2024Article
- A change inbioRxiv : the preprint server for biology · 2024Article
- Cereblon influences the timing of muscle differentiation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Using linkage logic theory to control dynamics of a gene regulatory network of a chordate embryo.Scientific reports · 2021Article
- Bilaterally Asymmetric Helical Myofibrils in Ascidian Tadpole Larvae.Frontiers in cell and developmental biology · 2021Article
- Review
- Article
- The Ciona myogenic regulatory factor functions as a typical MRF but possesses a novel N-terminus that is essential for activity.Developmental biology · 2019Article
- Review
- Controlling Cell Fate Specification System by Key Genes Determined from Network Structure.iScience · 2018Article
- Gene regulatory networks and cell lineages that underlie the formation of skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Rewiring of an ancestral Tbx1/10-Ebf-Mrf network for pharyngeal muscle specification in distinct embryonic lineages.Development (Cambridge, England) · 2016Article
- Regulation and evolution of cardiopharyngeal cell identity and behavior: insights from simple chordates.Current opinion in genetics & development · 2015Review
- Gene regulatory systems that control gene expression in the Ciona embryo.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2015Review
- Collier/OLF/EBF-dependent transcriptional dynamics control pharyngeal muscle specification from primed cardiopharyngeal progenitors.Developmental cell · 2014Article
- Patterns of positive selection of the myogenic regulatory factor gene family in vertebrates.PloS one · 2014Article
- Myogenesis in the sea urchin embryo: the molecular fingerprint of the myoblast precursors.EvoDevo · 2013Article
- Functional studies of the Ciona intestinalis myogenic regulatory factor reveal conserved features of chordate myogenesis.Developmental biology · 2013Article
- Purification of mitochondrial proteins HSP60 and ATP synthase from ascidian eggs: implications for antibody specificity.PloS one · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The activity of myogenic regulatory factor (MRF) genes is essential for vertebrate muscle development, whereas invertebrate muscle development is largely independent of MRF function. This difference indicates that myogenesis is controlled by distinct regulatory mechanisms in these two groups of animals. Here we used overexpression and gene knockdown to investigate the role in embryonic myogenesis of the single MRF gene of the invertebrate chordate Ciona intestinalis (Ci-MRF). Injection of Ci-MRF mRNA into eggs resulted in increased embryonic muscle-specific gene activity and revealed the myogenic activity of Ci-MRF by inducing the expression of four muscle marker genes, Acetylcholinesterase, Actin, Troponin I, and Myosin Light Chain in non-muscle lineages. Conversely, inhibiting Ci-MRF activity with antisense morpholinos down-regulated the expression of these genes. Consistent with the effects of morpholinos on muscle gene activity, larvae resulting from morpholino injection were paralyzed and their "muscle" cells lacked myofibrils. We conclude that Ci-MRF is required for larval tail muscle development and thus that an MRF-dependent myogenic regulatory network probably existed in the ancestor of tunicates and vertebrates. This possibility raises the question of whether the earliest myogenic regulatory networks were MRF-dependent or MRF-independent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.