ArticleBMC cancer2006
Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer.
Article in BMC cancer, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
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- Predicting novel biomarkers for early diagnosis and dynamic severity monitoring of human ulcerative colitis.Frontiers in genetics · 2024Article
- Comprehensive Analysis of METTLs (METTL1/13/18/21A/23/25/2A/2B/5/6/9) and Associated mRNA Risk Signature in Hepatocellular Carcinoma.Analytical cellular pathology (Amsterdam) · 2023Article
- A novel signature to predict the neoadjuvant chemotherapy response of bladder carcinoma: Results from a territory multicenter real-world study.Frontiers in genetics · 2022Article
- OMICfpp: a fuzzy approach for paired RNA-Seq counts.BMC genomics · 2019Article
- Common molecular markers between circulating tumor cells and blood exosomes in colorectal cancer: a systematic and analytical review.Cancer management and research · 2019Article
- Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer.Journal of inflammation research · 2018Article
- Downregulation of serum metabolite GTA-446 as a novel potential marker for early detection of colorectal cancer.British journal of cancer · 2017Article
- Systematic large-scale meta-analysis identifies a panel of two mRNAs as blood biomarkers for colorectal cancer detection.Oncotarget · 2016Article
- Small RNA sequencing-microarray analyses in Parkinson leukocytes reveal deep brain stimulation-induced splicing changes that classify brain region transcriptomes.Frontiers in molecular neuroscience · 2013Article
- Biology and significance of circulating and disseminated tumour cells in colorectal cancer.Langenbeck's archives of surgery · 2012Review
- Reduction of novel circulating long-chain fatty acids in colorectal cancer patients is independent of tumor burden and correlates with age.BMC gastroenterology · 2010Article
- The effects of globin on microarray-based gene expression analysis of mouse blood.Mammalian genome : official journal of the International Mammalian Genome Society · 2010Article
- Towards a more functional concept of causality in cancer research.International journal of molecular epidemiology and genetics · 2010Article
- Reduced levels of hydroxylated, polyunsaturated ultra long-chain fatty acids in the serum of colorectal cancer patients: implications for early screening and detection.BMC medicine · 2010Article
- A genomic approach to human autoimmune diseases.Annual review of immunology · 2010Review
- How the study of children with rheumatic diseases identified interferon-alpha and interleukin-1 as novel therapeutic targets.Immunological reviews · 2008Review
- Microarray-based identification of novel biomarkers in IL-1-mediated diseases.Current opinion in immunology · 2007Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions.
methodsIn this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription-polymerase chain reaction (RT-PCR).
resultsOur present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify.
conclusionThe design of new approaches to identify such markers is warranted.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.