ArticleThe EMBO journal2006
Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 79 citations in OpenAlex.
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- CK2β Is a Gatekeeper of Focal Adhesions Regulating Cell Spreading.Frontiers in molecular biosciences · 2022Article
- The polarity protein PARD3 and cancer.Oncogene · 2021Review
- Tight junctions and their regulation by non-coding RNAs.International journal of biological sciences · 2021Review
- The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.Nature communications · 2020Article
- Leader cell PLCγ1 activation during keratinocyte collective migration is induced by EGFR localization and clustering.Bioengineering & translational medicine · 2019Article
- The adherens junction-associated LIM domain protein Smallish regulates epithelial morphogenesis.The Journal of cell biology · 2018Article
- KIF22 coordinates CAR and EGFR dynamics to promote cancer cell proliferation.Science signaling · 2018Article
- Adherens Junction and E-Cadherin complex regulation by epithelial polarity.Cellular and molecular life sciences : CMLS · 2016Review
- Article
- Dual function of partitioning-defective 3 in the regulation of YAP phosphorylation and activation.Cell discovery · 2016Article
- Systematic analysis of copy number variants of a large cohort of orofacial cleft patients identifies candidate genes for orofacial clefts.Human genetics · 2016Article
- The Mammalian Blood-Testis Barrier: Its Biology and Regulation.Endocrine reviews · 2015Review
- Regulation of the cystic fibrosis transmembrane conductance regulator anion channel by tyrosine phosphorylation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Actin-interacting protein 1 controls assembly and permeability of intestinal epithelial apical junctions.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Use of mechanistic models to integrate and analyze multiple proteomic datasets.Biophysical journal · 2015Article
- Postembryonic lineages of the Drosophila brain: II. Identification of lineage projection patterns based on MARCM clones.Developmental biology · 2013Article
- Endocytosis and cancer.Cold Spring Harbor perspectives in biology · 2013Review
- ERK2-mediated phosphorylation of Par3 regulates neuronal polarization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Expression of partitioning defective 3 (Par-3) for predicting extrahepatic metastasis and survival with hepatocellular carcinoma.International journal of molecular sciences · 2013Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conserved polarity complex, comprising the partitioning-defective (Par) proteins Par3 and Par6, and the atypical protein kinase C, functions in various cell-polarization events and asymmetric cell divisions. However, little is known about whether and how external stimuli-induced signals may regulate Par3 function in epithelial cell polarity. Here, we found that Par3 was tyrosine phosphorylated through phosphoproteomic profiling of pervanadate-induced phosphotyrosine proteins. We also demonstrated that the tyrosine phosphorylation event induced by multiple growth factors including epidermal growth factor (EGF) was dependent on activation of Src family kinase (SFK) members c-Src and c-Yes. The tyrosine residue 1127 (Y1127) of Par3 was identified as the major EGF-induced phosphorylation site. Moreover, we found that Y1127 phosphorylation reduced the association of Par3 with LIM kinase 2 (LIMK2), thus enabling LIMK2 to regulate cofilin phosphorylation dynamics. Substitution of Y1127 for phenylalanine impaired the EGF-induced Par3 and LIMK2 dissociation and delayed epithelial tight junction (TJ) assembly considerably. Collectively, these data suggest a novel, phosphotyrosine-dependent fine-tuning mechanism of Par3 in epithelial TJ assembly controlled by the EGF receptor-SFK signaling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.