ArticleMolecular cell2006
A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment.
Article in Molecular cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Transcription remodeling and stalling by cisplatin-induced interstrand crosslinks.Nature chemical biology · 2026Article
- Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins.Communications chemistry · 2025Article
- Promoter-independent synthesis of chemically modified RNA by human DNA polymerase θ variants.RNA (New York, N.Y.) · 2023Article
- High-LET-Radiation-Induced Persistent DNA Damage Response Signaling and Gastrointestinal Cancer Development.Current oncology (Toronto, Ont.) · 2023Review
- Ebola Virus Produces Discrete Small Noncoding RNAs Independently of the Host MicroRNA Pathway Which Lack RNA Interference Activity in Bat and Human Cells.Journal of virology · 2020Article
- Identification of a Small Interface between the Methyltransferase and RNA Polymerase of NS5 that is Essential for Zika Virus Replication.Scientific reports · 2018Article
- Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent.Microbial cell factories · 2018Article
- 3' end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character-RNA-Seq analyses.Nucleic acids research · 2018Article
- Transcriptional fidelities of human mitochondrial POLRMT, yeast mitochondrial Rpo41, and phage T7 single-subunit RNA polymerases.The Journal of biological chemistry · 2017Article
- Article
- Deciphering the biology of porcine epidemic diarrhea virus in the era of reverse genetics.Virus research · 2016Review
- Studies on Transcriptional Incorporation of 5'-N-Triphosphates of 5'-Amino-5'-Deoxyribonucleosides.PloS one · 2016Article
- RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes.Nucleic acids research · 2015Article
- Unraveling the mechanistic features of RNA polymerase II termination by the 5'-3' exoribonuclease Rat1.Nucleic acids research · 2015Article
- T7 RNA polymerases backed up by covalently trapped proteins catalyze highly error prone transcription.The Journal of biological chemistry · 2012Article
- Templated nucleoside triphosphate binding to a noncatalytic site on RNA polymerase regulates transcription.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Comparative Study of Cyanobacterial and E. coli RNA Polymerases: Misincorporation, Abortive Transcription, and Dependence on Divalent Cations.Genetics research international · 2011Article
- Maintenance of RNA-DNA hybrid length in bacterial RNA polymerases.The Journal of biological chemistry · 2009Article
- Nucleic acid polymerases use a general acid for nucleotidyl transfer.Nature structural & molecular biology · 2009Article
- Differential blocking effects of the acetaldehyde-derived DNA lesion N2-ethyl-2'-deoxyguanosine on transcription by multisubunit and single subunit RNA polymerases.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Transcription errors by T7 RNA polymerase (RNAP) may occur as the result of a mechanism in which the template base two positions downstream of the 3' end of the RNA (the TSn+1 base) is utilized during two consecutive nucleotide-addition cycles. In the first cycle, misalignment of the template strand leads to incorporation of a nucleotide that is complementary to the TSn+1 base. In the second cycle, the template is realigned and the mismatched primer is efficiently extended, resulting in a substitution error. Proper organization of the transcription bubble is required for maintaining the correct register of the DNA template, as the presence of a complementary nontemplate strand opposite the TSn+1 base suppresses template misalignment. Our findings for T7 RNAP are in contrast to related DNA polymerases of the Pol I type, which fail to extend mismatches efficiently and generate predominantly deletion errors as a result of template-strand misalignment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.