ArticleMolecular biology of the cell2006
The monomeric clathrin assembly protein, AP180, regulates contractile vacuole size in Dictyostelium discoideum.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- VPS8D, a CORVET subunit, is required to maintain the contractile vacuole complex inbioRxiv : the preprint server for biology · 2023Article
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- The Polarized Redistribution of the Contractile Vacuole to the Rear of the Cell is Critical for Streaming and is Regulated by PI(4,5)P2-Mediated Exocytosis.Frontiers in cell and developmental biology · 2021Review
- Clathrin Light Chains: Not to Be Taken so Lightly.Frontiers in cell and developmental biology · 2021Review
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- The Molecular Physiopathogenesis of Islet Amyloidosis.Handbook of experimental pharmacology · 2018Review
- Conservation and divergence within the clathrin interactome of Trypanosoma cruzi.Scientific reports · 2016Article
- ENTH and ANTH domain proteins participate in AP2-independent clathrin-mediated endocytosis.Journal of cell science · 2015Article
- Unconventional functions for clathrin, ESCRTs, and other endocytic regulators in the cytoskeleton, cell cycle, nucleus, and beyond: links to human disease.Cold Spring Harbor perspectives in biology · 2014Review
- Distinct internalization pathways of human amylin monomers and its cytotoxic oligomers in pancreatic cells.PloS one · 2013Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium.Journal of cell science · 2012Article
- A single β adaptin contributes to AP1 and AP2 complexes and clathrin function in Dictyostelium.Traffic (Copenhagen, Denmark) · 2012Article
- Article
- Regulation of Hip1r by epsin controls the temporal and spatial coupling of actin filaments to clathrin-coated pits.Journal of cell science · 2010Article
- Roles of an unconventional protein kinase and myosin II in amoeba osmotic shock responses.Traffic (Copenhagen, Denmark) · 2009Article
- AP180-mediated trafficking of Vamp7B limits homotypic fusion of Dictyostelium contractile vacuoles.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
AP180, one of many assembly proteins and adaptors for clathrin, stimulates the assembly of clathrin lattices on membranes, but its unique contribution to clathrin function remains elusive. In this study we identified the Dictyostelium discoideum ortholog of the adaptor protein AP180 and characterized a mutant strain carrying a deletion in this gene. Imaging GFP-labeled AP180 showed that it localized to punctae at the plasma membrane, the contractile vacuole, and the cytoplasm and associated with clathrin. AP180 null cells did not display defects characteristic of clathrin mutants and continued to localize clathrin punctae on their plasma membrane and within the cytoplasm. However, like clathrin mutants, AP180 mutants, were osmosensitive. When immersed in water, AP180 null cells formed abnormally large contractile vacuoles. Furthermore, the cycle of expansion and contraction for contractile vacuoles in AP80 null cells was twice as long as that of wild-type cells. Taken together, our results suggest that AP180 plays a unique role as a regulator of contractile vacuole morphology and activity in Dictyostelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.