ArticleThe British journal of ophthalmology2007
Comparison of an rRNA-based and DNA-based nucleic acid amplification test for the detection of Chlamydia trachomatis in trachoma.
Article in The British journal of ophthalmology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- The efficacy of oral azithromycin in clearing ocular chlamydia: mathematical modeling from a community-randomized trachoma trial.Epidemics · 2014Trial
- Diagnostic characteristics of tests for ocular Chlamydia after mass azithromycin distributions.Investigative ophthalmology & visual science · 2012Trial
- How reliable are tests for trachoma?--a latent class approach.Investigative ophthalmology & visual science · 2011Trial
- Comparison of Methods for Assessing Chlamydia trachomatis Transmission Intensity: A Systematic Review.The American journal of tropical medicine and hygiene · 2026Article
- Comparison between DNA- and RNA-based nucleic acid amplification tests for detecting Mycoplasma pneumoniae in pediatric specimens.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Use of viability PCR for detection of live Chlamydia trachomatis in clinical specimens.Frontiers in reproductive health · 2023Article
- Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Ocular Infections.Clinical microbiology reviews · 2021Review
- Comparison of rRNA-based and DNA-based nucleic acid amplifications for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Ureaplasma urealyticum in urogenital swabs.BMC infectious diseases · 2018Article
- The Relationship between Active Trachoma and Ocular Chlamydia trachomatis Infection before and after Mass Antibiotic Treatment.PLoS neglected tropical diseases · 2016Review
- Low Prevalence of Conjunctival Infection with Chlamydia trachomatis in a Treatment-Naïve Trachoma-Endemic Region of the Solomon Islands.PLoS neglected tropical diseases · 2016Article
- Development and evaluation of a next-generation digital PCR diagnostic assay for ocular Chlamydia trachomatis infections.Journal of clinical microbiology · 2013Article
- Using a nonparametric multilevel latent Markov model to evaluate diagnostics for trachoma.American journal of epidemiology · 2013Article
- Trachoma: an update on prevention, diagnosis, and treatment.Current opinion in ophthalmology · 2012Review
- Chlamydial infection during trachoma monitoring: are the most difficult-to-reach children more likely to be infected?Tropical medicine & international health : TM & IH · 2012Review
- Direct fluorescent antibody assay and polymerase chain reaction for the detection of Chlamydia trachomatis in patients with vernal keratoconjunctivitis.Clinics (Sao Paulo, Brazil) · 2011Article
- Molecular Diagnosis of Sexually Transmitted Chlamydia trachomatis in the United States.ISRN obstetrics and gynecology · 2011Article
- Trachoma rapid assessments in Unity and Northern Bahr-el-Ghazal States, Southern Sudan.PloS one · 2010Article
- Reports on the prevalence of clinical conditions are more convincing when supported by objective evidence.PLoS neglected tropical diseases · 2008Article
- rRNA-based tests for chlamydial infection in trachoma.The British journal of ophthalmology · 2007Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND/
aimThe World Health Organisation (WHO) hopes to achieve global elimination of trachoma, still the leading cause of preventable blindness worldwide, in part through mass antibiotic treatment. DNA-based nucleic acid amplification tests (NAATs) are currently used to evaluate the success of treatment programmes by measuring the prevalence of C trachomatis infection. Some believe that newer ribosomal RNA (rRNA)-based tests may be much more sensitive since bacterial rRNA is present in amounts up to 10 000 times that of genomic DNA. Others believe that rRNA-based tests are instead less sensitive but more specific, due to the presence of dead or subviable organisms that the test may not detect. This study compares an rRNA-based test to a DNA-based test for the detection of ocular C trachomatis infection in children living in trachoma-endemic villages.
methodsAn rRNA-based amplification test and DNA-based polymerase chain reaction (PCR) were performed on swab specimens taken from the right upper tarsal conjunctiva of 56 children aged 0-10 years living in two villages in Amhara, Ethiopia.
resultsThe rRNA-based test detected ocular C trachomatis infection in 35 (63%) subjects compared with 22 (39%) detected by PCR (McNemar's test, p = 0.0002). The rRNA-based test gave positive results for all subjects that were positive by PCR, and also detected infection in 13 (23%) additional subjects.
conclusionThe rRNA-based test appears to have significantly greater sensitivity than PCR for the detection of ocular chlamydial infection in children in trachoma-endemic villages. Using the rRNA-based test, we may be able to detect infection that was previously missed with PCR. Past studies using DNA-based tests to assess prevalence of infectious trachoma following antibiotic treatment may have underestimated the true prevalence of infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.