ArticleProceedings of the National Academy of Sciences of the United States of America1991
The use of sarkosyl in generating soluble protein after bacterial expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 130 citations in OpenAlex.
- Sodium Dodecyl Sulfate Analogs as a Potential Molecular Biology Reagent.Current issues in molecular biology · 2024Review
- Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography.Microbial cell factories · 2023Article
- Development and Validation of ScriptTaq COVID PCR: An In-House Multiplex rRT-PCR for Low-Cost Detection.Current issues in molecular biology · 2022Article
- A solution to the long-standing problem of actin expression and purification.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Evaluation of factors influencing expression and extraction of recombinant bacteriophage endolysins in Escherichia coli.Microbial cell factories · 2022Article
- A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis.mSystems · 2021Article
- Studies on the Structure and Properties of Membrane Phospholipase AMolecules (Basel, Switzerland) · 2021Article
- Purification of Tag-FreeBiochemistry · 2019Article
- A high-throughput pipeline for the production of synthetic antibodies for analysis of ribonucleoprotein complexes.RNA (New York, N.Y.) · 2016Article
- Characterization of Outer Membrane Proteome of Akkermansia muciniphila Reveals Sets of Novel Proteins Exposed to the Human Intestine.Frontiers in microbiology · 2016Article
- Use of anionic denaturing detergents to purify insoluble proteins after overexpression.BMC biotechnology · 2012Article
- Quaternary structure of KATP channel SUR2A nucleotide binding domains resolved by synchrotron radiation X-ray scattering.Journal of structural biology · 2010Article
- Article
- Interaction with CagF is required for translocation of CagA into the host via the Helicobacter pylori type IV secretion system.Infection and immunity · 2006Article
- Histidine-tagged wild-type yeast actin: its properties and use in an approach for obtaining yeast actin mutants.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A cytoplasmic protein, bystin, interacts with trophinin, tastin, and cytokeratin and may be involved in trophinin-mediated cell adhesion between trophoblast and endometrial epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Unconventional myosins in inner-ear sensory epithelia.The Journal of cell biology · 1997Article
- The IE2 regulatory protein of human cytomegalovirus induces expression of the human transforming growth factor beta1 gene through an Egr-1 binding site.Journal of virology · 1996Article
- Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Regulation of transforming growth factor-beta 1 expression by the hepatitis B virus (HBV) X transactivator. Role in HBV pathogenesis.The Journal of clinical investigation · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Actin, like many other proteins, is highly insoluble after expression in Escherichia coli. In order to understand the origin of insoluble aggregates, we asked whether morphological inclusions were always correlated with insolubility. The strain expressing actin was compared to one that expresses part of the myosin tail; the latter strain yields soluble protein after various cell lysis or disruption procedures. Morphological inclusions were observed in both strains, indicating there is no obligate relationship between solubility and inclusions. Studies presented here suggest that extreme insolubility results from coaggregation of the actin with bacterial outer membrane components upon bacterial lysis. The properties of the outer membrane have been exploited in the development of nondenaturing procedures that yield soluble actin. One procedure involves the disruption of coaggregates with sarkosyl detergent (N-laurylsarcosine); another prevents the formation of coaggregates by lysing in the presence of sarkosyl. These methods may be useful for other proteins that become insoluble after bacterial expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.