ArticleCurrent microbiology2006
A thermostable alpha-galactosidase from Lactobacillus fermentum CRL722: genetic characterization and main properties.
Article in Current microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
- Prebiotic Effects of α- and β-Galactooligosaccharides: The Structure-Function Relation.Molecules (Basel, Switzerland) · 2025Review
- Unraveling the mechanism of raffinose utilization in3 Biotech · 2022Article
- Advances in research on solid-state fermented feed and its utilization: The pioneer of private customization for intestinal microorganisms.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- Cloning and expression of a novel α-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties.PloS one · 2020Article
- Effect ofInternational journal of food science · 2020Article
- Characterization of Properties and Transglycosylation Abilities of Recombinant α-Galactosidase from Cold-Adapted Marine BacteriumMarine drugs · 2018Article
- A β-mannan utilization locus in Bacteroides ovatus involves a GH36 α-galactosidase active on galactomannans.FEBS letters · 2016Article
- Purification and characterisation of intracellular alpha-galactosidases from Acinetobacter sp.3 Biotech · 2015Article
- Thermostability of Probiotics and Their α -Galactosidases and the Potential for Bean Products.Biotechnology research international · 2014Article
- Metabolism of oligosaccharides and starch in lactobacilli: a review.Frontiers in microbiology · 2012Article
- Thermostable enzymes as biocatalysts in the biofuel industry.Advances in applied microbiology · 2010Review
- Ability of Lactobacillus fermentum to overcome host alpha-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya alpha-galacto-oligosaccharides.BMC microbiology · 2008Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alpha-galactosidase (alpha-Gal) enzyme, which is encoded by the melA gene hydrolyzes alpha-1,6 galactoside linkages found in sugars, such as raffinose and stachyose. These alpha-galacto-oligosaccharides (alpha-GOS), which are found in large quantities in vegetables, such as soy, can cause gastrointestinal disorders in sensitive individuals because monogastric animals (including humans) do not posses alpha-Gal in the gut. The use of microbial alpha-Gal is a promising alternative to eliminate alpha-GOS in soy-derived products. Using degenerate primers, the melA gene from Lactobacillus (L.) fermentum CRL722 was identified. The complete genomic sequence of melA (2223 bp), and of the genes flanking melA, were obtained using a combination of polymerase chain reaction-based techniques, and showed strong similarities with the alpha-Gal gene of thermophilic microorganisms. The alpha-Gal gene from L. fermentum CRL722 was cloned and the protein purified from cell-free extracts of the native and recombinant strains using various techniques (ion exchange chromatography, salt precipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and ultra-filtration); Its main biochemical properties were determined. The enzyme was active at moderately high temperatures (55 degrees C) and stable at wide ranges of temperatures and pH. The thermostable alpha-Gal from L. fermentum CRL722 could thus be used for technological applications, such as the removal of alpha-GOS found in soy products. The complete melA gene could also be inserted in other micro-organisms, that can survive in the harsh conditions of the gut to degrade alpha-GOS in situ. Both strategies would improve the overall acceptability of soy-derived products by improving their nutritional value.
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