ArticleMediators of inflammation2006
Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro.
Article in Mediators of inflammation, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 17 citations in OpenAlex.
- Modular and automated synthesis of oligonucleotide-small molecule conjugates for cathepsin B mediated traceless release of payloads.RSC chemical biology · 2024Article
- [Effects of neutrophils alkaline phosphatase on functions of neutrophils in vitro].Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2016Article
- Seasonal changes in activities of human neutrophils in vitro.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2012Article
- Extracellular ATP and P2 receptors are required for IL-8 to induce neutrophil migration.Cytokine · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have proposed the use of the fluorimetric method with 4-methylumbelliferyl phosphate (4-MUP) specific substrate for the alkaline phosphatase determination in the neutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkaline phosphatase (NAP) activity evaluation is reliable to quantify neutrophil adhesion at a wide range of cell numbers (10(4)-10(6)). The results obtained by fluorimetric NAP activity test correlate to the results of adherence evaluated using the MTT reduction assay. The fluorimetric NAP activity test may be applied for resting as well as activated neutrophils without the risk of the activators interferences into the test. The alkaline phosphatase survey with the use of 4-MUP substrate is recommended herein as a sensitive, repeatable, simple, and reliable method of the neutrophil adherence determination in vitro.
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Registered trials
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