ArticleMolecular and cellular biology2006
ZP2 and ZP3 traffic independently within oocytes prior to assembly into the extracellular zona pellucida.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- Aging disrupts spatiotemporal coordination in the cycling murine ovary.Nature aging · 2026Article
- Furin-Mediated Cleavage of Zona Pellucida Proteins Is Essential for Oocyte Development.MedComm · 2025Article
- Dominant follicle-targeted nanocarriers for GH delivery to alleviate premature ovarian insufficiency.Materials today. Bio · 2025Article
- Intracellular fraction of zona pellucida protein 3 is required for the oocyte-to-embryo transition in mice.Molecular human reproduction · 2023Article
- Germ Granules in Animal Oogenesis.Journal of developmental biology · 2022Review
- Zona Pellucida Genes and Proteins: Essential Players in Mammalian Oogenesis and Fertility.Genes · 2021Review
- New Insights into the Mammalian Egg Zona Pellucida.International journal of molecular sciences · 2021Review
- Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.PloS one · 2021Article
- Mammalian egg coat modifications and the block to polyspermy.Molecular reproduction and development · 2020Review
- Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.Nature communications · 2019Article
- Novel mutations in ZP1, ZP2, and ZP3 cause female infertility due to abnormal zona pellucida formation.Human genetics · 2019Article
- ZP2 pathogenic variants cause in vitro fertilization failure and female infertility.Genetics in medicine : official journal of the American College of Medical Genetics · 2019Article
- Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility.Human genetics · 2017Article
- ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis.Scientific reports · 2017Article
- Mutant ZP1 in familial infertility.The New England journal of medicine · 2014Article
- The molecular basis of gamete recognition in mice and humans.Molecular human reproduction · 2013Review
- Generation of a germ cell-specific mouse transgenic CHERRY reporter, Sohlh1-mCherryFlag.Genesis (New York, N.Y. : 2000) · 2013Article
- ZP2 and ZP3 cytoplasmic tails prevent premature interactions and ensure incorporation into the zona pellucida.Journal of cell science · 2011Article
- Mouse early oocytes are transiently polar: three-dimensional and ultrastructural analysis.Experimental cell research · 2008Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The extracellular zona pellucida surrounds mammalian eggs and mediates taxon-specific sperm-egg recognition at fertilization. In mice, the zona pellucida is composed of three glycoproteins, but the presence of ZP2 and ZP3 is sufficient to form a biologically functional structure. Each zona pellucida glycoprotein is synthesized in growing oocytes and traffics through the endomembrane system to the cell surface, where it is released from a transmembrane domain and assembled into the insoluble zona pellucida matrix. ZP2 and ZP3 colocalize in the endoplasmic reticulum and in 1- to 5-microm post-Golgi structures comprising multivesicular aggregates (MVA), but a coimmunoprecipitation assay does not detect physical interactions. In addition, ZP2 traffics normally in growing oocytes in the absence of ZP3 or if ZP3 has been mutated to prevent incorporation into the zona pellucida matrix, complementing earlier studies indicating the independence of ZP3 secretion in Zp2 null mice. N glycosylation has been implicated in correct protein folding and intracellular trafficking of secreted proteins. Although ZP3 contain five N-glycans, enhanced green fluorescent protein-tagged ZP3 lacking N glycosylation sites is present in MVA and is incorporated into the zona pellucida matrix of transgenic mice. Thus, ZP2 secretion is seemingly unaffected by ZP3 lacking N-glycans. Taken together, these observations indicate that ZP2 and ZP3 traffic independently through the oocyte prior to assembly into the zona pellucida.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.