ArticleJournal of molecular biology2007
RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 71 citations in OpenAlex.
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- Insights into the biosynthesis of septacidin l-heptosamine moiety unveils a VOC family sugar epimerase.Acta pharmaceutica Sinica. B · 2023Article
- Rhamnose-Containing Compounds: Biosynthesis and Applications.Molecules (Basel, Switzerland) · 2022Review
- Spinning sugars in antigen biosynthesis: characterization of the Coxiella burnetii and Streptomyces griseus TDP-sugar epimerases.The Journal of biological chemistry · 2022Article
- Biosynthesis of d-Biochemistry · 2021Article
- Biosynthetic access to the rare antiarose sugarChemical science · 2020Article
- Discovery of an RmlC/D fusion protein in the microalgaThe Journal of biological chemistry · 2019Article
- Streptococcal dTDP-L-rhamnose biosynthesis enzymes: functional characterization and lead compound identification.Molecular microbiology · 2019Article
- Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose 3,5-epimerase (RfbC).Acta crystallographica. Section F, Structural biology communications · 2017Article
- Pac13 is a Small, Monomeric Dehydratase that Mediates the Formation of the 3'-Deoxy Nucleoside of Pacidamycins.Angewandte Chemie (International ed. in English) · 2017Article
- Non-enzymatic pyridine ring formation in the biosynthesis of the rubrolone tropolone alkaloids.Nature communications · 2016Article
- Bacterial glycobiology: rhamnose-containing cell wall polysaccharides in Gram-positive bacteria.FEMS microbiology reviews · 2016Review
- A Putative ABC Transporter Permease Is Necessary for Resistance to Acidified Nitrite and EDTA in Pseudomonas aeruginosa under Aerobic and Anaerobic Planktonic and Biofilm Conditions.Frontiers in microbiology · 2016Article
- Biochemical studies on WbcA, a sugar epimerase from Yersinia enterocolitica.Protein science : a publication of the Protein Society · 2015Article
- The flagellin FliC of Clostridium difficile is responsible for pleiotropic gene regulation during in vivo infection.PloS one · 2014Article
- Comparison of predicted epimerases and reductases of the Campylobacter jejuni D-altro- and L-gluco-heptose synthesis pathways.The Journal of biological chemistry · 2013Article
- Detection and characterization of megasatellites in orthologous and nonorthologous genes of 21 fungal genomes.Eukaryotic cell · 2013Article
- Effects of histidine protonation and rotameric states on virtual screening of M. tuberculosis RmlC.Journal of computer-aided molecular design · 2013Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The striking feature of carbohydrates is their constitutional, conformational and configurational diversity. Biology has harnessed this diversity and manipulates carbohydrate residues in a variety of ways, one of which is epimerization. RmlC catalyzes the epimerization of the C3' and C5' positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose. RmlC is the third enzyme of the rhamnose pathway, and represents a validated anti-bacterial drug target. Although several structures of the enzyme have been reported, the mechanism and the nature of the intermediates have remained obscure. Despite its relatively small size (22 kDa), RmlC catalyzes four stereospecific proton transfers and the substrate undergoes a major conformational change during the course of the transformation. Here we report the structure of RmlC from several organisms in complex with product and product mimics. We have probed site-directed mutants by assay and by deuterium exchange. The combination of structural and biochemical data has allowed us to assign key residues and identify the conformation of the carbohydrate during turnover. Clear knowledge of the chemical structure of RmlC reaction intermediates may offer new opportunities for rational drug design.
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