ArticleBiochemical pharmacology2007
Evidence for significant contribution of a newly identified monoamine transporter (PMAT) to serotonin uptake in the human brain.
Article in Biochemical pharmacology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 71 citations in OpenAlex.
- PMAT enhances sexual dimorphism of fear behaviors and facilitates female mice's generalized contextual fear extinction.Frontiers in neuroscience · 2026Article
- Review
- All the brain's a stage for serotonin: the forgotten story of serotonin diffusion across cell membranes.Proceedings. Biological sciences · 2022Review
- Review of Transporter Substrate, Inhibitor, and Inducer Characteristics of Cladribine.Clinical pharmacokinetics · 2021Review
- Interaction Profiles of Central Nervous System Active Drugs at Human Organic Cation Transporters 1-3 and Human Plasma Membrane Monoamine Transporter.International journal of molecular sciences · 2021Article
- Implantable aptamer-field-effect transistor neuroprobes for in vivo neurotransmitter monitoring.Science advances · 2021Article
- Inflammation-Induced Histamine Impairs the Capacity of Escitalopram to Increase Hippocampal Extracellular Serotonin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Organic Cation Transporter Expression and Function in the CNS.Handbook of experimental pharmacology · 2021Article
- General Overview of Organic Cation Transporters in Brain.Handbook of experimental pharmacology · 2021Article
- Brain Plasma Membrane Monoamine Transporter in Health and Disease.Handbook of experimental pharmacology · 2021Article
- Serotonin Transporter and Plasma Membrane Monoamine Transporter Are Necessary for the Antidepressant-Like Effects of Ketamine in Mice.International journal of molecular sciences · 2020Article
- Embracing diversity in the 5-HT neuronal system.Nature reviews. Neuroscience · 2019Review
- Comparative analysis of novel decynium-22 analogs to inhibit transport by the low-affinity, high-capacity monoamine transporters, organic cation transporters 2 and 3, and plasma membrane monoamine transporter.European journal of pharmacology · 2019Article
- Serotonin Syndrome: Pathophysiology, Clinical Features, Management, and Potential Future Directions.International journal of tryptophan research : IJTR · 2019Review
- Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine.Frontiers in cellular neuroscience · 2018Article
- Equilibrative nucleoside transporters-A review.Nucleosides, nucleotides & nucleic acids · 2017Review
- The plasma membrane monoamine transporter (PMAT): Structure, function, and role in organic cation disposition.Clinical pharmacology and therapeutics · 2016Review
- Polyspecific organic cation transporters and their impact on drug intracellular levels and pharmacodynamics.Pharmacological research · 2016Review
- Potent and Selective Inhibition of Plasma Membrane Monoamine Transporter by HIV Protease Inhibitors.Drug metabolism and disposition: the biological fate of chemicals · 2015Article
- An immunohistochemical analysis of SERT in the blood-brain barrier of the male rat brain.Histochemistry and cell biology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The high affinity serotonin transporter (SERT) constitutes the principal pathway for removal of serotonin (5-HT) from extracellular fluid of brain, but evidence indicates that other transporters may also be involved in this process. We recently reported the cloning of a novel plasma membrane monoamine transporter (PMAT), which is abundantly expressed in the human brain and avidly transports 5-HT [Engel K, Zhou M, Wang J. Identification and characterization of a novel monoamine transporter in the human brain. J Biol Chem 2004;279:50042-9]. In this study, we evaluated whether PMAT contributes to total human brain uptake of 5-HT using a hybrid depletion approach in Xenopus laevis oocytes. We also examined whether PMAT interacts with selective serotonin reuptake inhibitors (SSRIs) using MDCK cells stably expressing recombinant human PMAT. Microinjection of total human brain poly(A)(+) mRNA into oocytes elicited approximately 2.5-3-fold increase in 5-HT uptake. Pre-hybridization of poly(A)(+) mRNA with PMAT or SERT antisense oligonucleotides significantly reduced mRNA-induced 5-HT uptake. An additive inhibitory effect was observed when poly(A)(+) mRNA was co-hybridized with both PMAT and SERT antisense oligonucleotides. In contrast, mRNA-induced 5-HT uptake was not affected by pre-hybridization with sense oligonucleotides. These data suggest that functional transcripts of PMAT are present in the human brain, and the PMAT transporter may be significantly involved in brain uptake of 5-HT. All five tested SSRIs inhibited PMAT with IC(50) values ranging from 11 to 116 microM, which are much greater than clinically encountered concentrations, suggesting that PMAT activity is minimally affected by SSRI therapies.
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