ReviewMethods in enzymology2006
Micro-imaging of amyloid in mice.
Review in Methods in enzymology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Late-stage labeling of diverse peptides and proteins with iodine-125.Journal of pharmaceutical analysis · 2025Review
- Preclinical evaluation of Tc-99m p5+14 peptide for SPECT detection of cardiac amyloidosis.PloS one · 2024Article
- Secondary structure propensity and chirality of the amyloidophilic peptide p5 and its analogues impacts ligand binding -Biochemistry and biophysics reports · 2016Article
- The pattern recognition reagents RAGE VC1 and peptide p5 share common binding sites and exhibit specific reactivity with AA amyloid in mice.Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis · 2016Article
- Preclinical Validation of the Heparin-Reactive Peptide p5+14 as a Molecular Imaging Agent for Visceral Amyloidosis.Molecules (Basel, Switzerland) · 2015Article
- Article
- Peptide p5 binds both heparinase-sensitive glycosaminoglycans and fibrils in patient-derived AL amyloid extracts.Biochemical and biophysical research communications · 2013Article
- A novel method for quantifying peripheral tissue amyloid load by using the radiolabeled amyloidophilic peptide, p5.Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis · 2013Article
- A binding-site barrier affects imaging efficiency of high affinity amyloid-reactive peptide radiotracers in vivo.PloS one · 2013Article
- Comparative analysis of peptide p5 and serum amyloid P component for imaging AA amyloid in mice using dual-isotope SPECT.Molecular imaging and biology · 2012Article
- Serum amyloid a protein in clinical cancer diagnosis.Pathology oncology research : POR · 2012Article
- Molecular imaging of amyloidosis: will the heart be the next target after the brain?Current cardiology reports · 2012Review
- AL amyloid imaging and therapy with a monoclonal antibody to a cryptic epitope on amyloid fibrils.PloS one · 2012Article
- SPECT imaging of peripheral amyloid in mice by targeting hyper-sulfated heparan sulfate proteoglycans with specific scFv antibodies.Nuclear medicine and biology · 2012Article
- Development and evaluation of agents for targeting visceral amyloid.Tijdschrift voor nu[c]leaire geneeskunde · 2011Article
- In vivo molecular imaging of peripheral amyloidosis using heparin-binding peptides.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Generation and characterization of anti-AA amyloid-specific monoclonal antibodies.Frontiers in immunology · 2011Article
- The heparan sulfate motif (GlcNS6S-IdoA2S)3, common in heparin, has a strict topography and is involved in cell behavior and disease.The Journal of biological chemistry · 2010Article
- Quantitative tomography of early-onset spontaneous AA amyloidosis in interleukin 6 transgenic mice.Comparative medicine · 2008Article
- Disruption of chromodomain helicase DNA binding protein 2 (CHD2) causes scoliosis.American journal of medical genetics. Part A · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Scintigraphic imaging of radioiodinated serum amyloid P-component is a proven method for the clinical detection of peripheral amyloid deposits (Hawkins et al., 1990). However, the inability to perform comparably high-resolution studies in experimental animal models of amyloid disease has impacted not only basic studies into the pathogenesis of amyloidosis but also in the preclinical in vivo evaluation of potential anti-amyloid therapeutic agents. We have developed microimaging technologies, implemented novel computational methods, and established protocols to generate high-resolution images of amyloid deposits in mice. (125)I-labeled serum amyloid P component (SAP) and an amyloid-fibril reactive murine monoclonal antibody (designated 11-1F4) have been used successfully to acquire high-resolution single photon emission computed tomographic (SPECT) images that, when fused with x-ray computed tomographic (CT) data, have provided precise anatomical localization of secondary (AA) and primary (AL) amyloid deposits in mouse models of these diseases. This chapter will provide detailed protocols for the radioiodination and purification of amyloidophilic proteins and the generation of mouse models of AA and AL amyloidosis. A brief description of the available hardware and the parameters used to acquire high-resolution microSPECT and CT images is presented, and the tools used to perform image reconstruction and visualization that permit the analysis and presentation of image data are discussed. Finally, we provide established methods for measuring organ- and tissue-specific activities with which to corroborate the microSPECT and CT images.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.