ArticleThe Journal of general physiology2006
Cooperative gating between single HCN pacemaker channels.
Article in The Journal of general physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- Structure mirroring function: What's the 'matter' with the funny current?The Journal of physiology · 2026Review
- Subunit-specific conductance of single homomeric and heteromeric HCN pacemaker channels at femtosiemens resolution.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structural basis for hyperpolarization-dependent opening of human HCN1 channel.Nature communications · 2024Article
- Missing validation of key features in HCN single-channel recordings.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Palmitoylation regulates the magnitude of HCN4-mediated currents in mammalian cells.Frontiers in physiology · 2023Article
- Physics of mechanotransduction by Piezo ion channels.The Journal of general physiology · 2022Review
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Weak Cation Selectivity in HCN Channels Results From KFunction (Oxford, England) · 2022Article
- Cooperativity of KScience advances · 2020Article
- Clusters of cooperative ion channels enable a membrane-potential-based mechanism for short-term memory.eLife · 2020Article
- Axonal Computations.Frontiers in cellular neuroscience · 2019Review
- Article
- Patch-clamp fluorometry-based channel counting to determine HCN channel conductance.The Journal of general physiology · 2016Article
- Intramembrane congestion effects on lysenin channel voltage-induced gating.European biophysics journal : EBJ · 2016Article
- Action potential initiation in a multi-compartmental model with cooperatively gating Na channels in the axon initial segment.Journal of computational neuroscience · 2015Article
- What is the most realistic single-compartment model of spike initiation?PLoS computational biology · 2015Review
- Distribution and function of HCN channels in the apical dendritic tuft of neocortical pyramidal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Human slack potassium channel mutations increase positive cooperativity between individual channels.Cell reports · 2014Article
- Family of prokaryote cyclic nucleotide-modulated ion channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- cAMP control of HCN2 channel Mg2+ block reveals loose coupling between the cyclic nucleotide-gating ring and the pore.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
HCN pacemaker channels (I(f), I(q), or I(h)) play a fundamental role in the physiology of many excitable cell types, including cardiac myocytes and central neurons. While cloned HCN channels have been studied extensively in macroscopic patch clamp experiments, their extremely small conductance has precluded single channel analysis to date. Nevertheless, there remain fundamental questions about HCN gating that can be resolved only at the single channel level. Here we present the first detailed single channel study of cloned mammalian HCN2. Excised patch clamp recordings revealed discrete hyperpolarization-activated, cAMP-sensitive channel openings with amplitudes of 150-230 fA in the activation voltage range. The average conductance of these openings was approximately 1.5 pS at -120 mV in symmetrical 160 mM K(+). Some traces with multiple channels showed unusual gating behavior, characterized by a variable long delay after a voltage step followed by runs of openings. Noise analysis on macroscopic currents revealed fluctuations whose magnitudes were systematically larger than predicted from the actual single channel current size, consistent with cooperativity between single HCN channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.