ArticleEukaryotic cell2006
Transcriptional regulation of MDR1, encoding a drug efflux determinant, in fluconazole-resistant Candida albicans strains through an Mcm1p binding site.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- The Microevolution of Antifungal Drug Resistance in Pathogenic Fungi.Microorganisms · 2023Review
- Post-transcriptional control of antifungal resistance in human fungal pathogens.Critical reviews in microbiology · 2023Review
- Overcoming Drug Resistance in a ClinicalAntibiotics (Basel, Switzerland) · 2023Article
- Experimental and in-host evolution of triazole resistance in human pathogenic fungi.Frontiers in fungal biology · 2022Review
- Update on Antifungal Drug Resistance.Current clinical microbiology reports · 2015Article
- Induction of Candida albicans drug resistance genes by hybrid zinc cluster transcription factors.Antimicrobial agents and chemotherapy · 2015Article
- Mechanisms of Antifungal Drug Resistance.Cold Spring Harbor perspectives in medicine · 2014Review
- Inducible and constitutive activation of two polymorphic promoter alleles of the Candida albicans multidrug efflux pump MDR1.Antimicrobial agents and chemotherapy · 2012Article
- Antifungal resistance and new strategies to control fungal infections.International journal of microbiology · 2012Article
- An MDR1 promoter allele with higher promoter activity is common in clinically isolated strains of Candida albicans.Molecular genetics and genomics : MGG · 2011Article
- Transcriptional profiling of azole-resistant Candida parapsilosis strains.Antimicrobial agents and chemotherapy · 2011Article
- Differential requirement of the transcription factor Mcm1 for activation of the Candida albicans multidrug efflux pump MDR1 by its regulators Mrr1 and Cap1.Antimicrobial agents and chemotherapy · 2011Article
- Regulation of efflux pump expression and drug resistance by the transcription factors Mrr1, Upc2, and Cap1 in Candida albicans.Antimicrobial agents and chemotherapy · 2011Article
- Calcineurin signaling and membrane lipid homeostasis regulates iron mediated multidrug resistance mechanisms in Candida albicans.PloS one · 2011Article
- The zinc cluster transcription factor Ahr1p directs Mcm1p regulation of Candida albicans adhesion.Molecular microbiology · 2011Article
- Xenobiotic efflux in bacteria and fungi: a genomics update.Advances in enzymology and related areas of molecular biology · 2011Review
- Efflux in fungi: la pièce de résistance.PLoS pathogens · 2009Review
- Identification of the Candida albicans Cap1p regulon.Eukaryotic cell · 2009Article
- A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans.BMC genomics · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Constitutive, high-level transcription of the gene encoding the drug efflux facilitator Mdr1p is commonly observed in laboratory and clinical strains of Candida albicans that are resistant to the antifungal drug fluconazole (FLC). In five independently isolated FLC(R) laboratory strains, introduction of a wild-type MDR1 promoter fragment fused to the yeast enhanced green fluorescent protein (yEGFP) reporter gene resulted in high-level expression of GFP, demonstrating that overexpression of MDR1 is dependent on a trans-acting factor. This study identified a 35-bp MDR1 promoter element, termed the MDRE, that mediates high-level MDR1 transcription. When inserted into a heterologous promoter, the MDRE was sufficient to mediate high-level expression of the yEGFP reporter gene specifically in MDR1 trans-activation strains. The MDRE promoted transcription in an orientation-independent and dosage-dependent manner. Deletion of the MDRE in the full-length promoter did not abolish MDR1 trans-activation, indicating that elements upstream of the MDRE also contribute to transcription of MDR1 in these overexpression strains. Analysis of the MDRE sequence indicated that it contains an Mcm1p binding site very similar in organization to the site seen upstream of the Saccharomyces cerevisiae MFA1 and STE2 genes. Electrophoretic mobility shift analysis demonstrated that both wild-type, FLC-sensitive and MDR1 trans-activated, FLC-resistant strains contain a factor that binds the MDRE. Depletion of Mcm1p, by use of a strain in which MCM1 expression is under the control of a regulated promoter (44), resulted in a loss of MDRE binding activity. Thus, the general transcription factor Mcm1p participates in the regulation of MDR1 expression.
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Registered trials
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