ArticleApplied and environmental microbiology2006
Genetic manipulation of Prochlorococcus strain MIT9313: green fluorescent protein expression from an RSF1010 plasmid and Tn5 transposition.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Light and carbon: Synthetic biology toward new cyanobacteria-based living biomaterials.Materials today. Bio · 2023Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Review
- The role of the cytoskeletal proteins MreB and FtsZ in multicellular cyanobacteria.FEBS open bio · 2020Article
- Article
- Toward a genetic system in the marine cyanobacteriumAccess microbiology · 2020Article
- Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting.iScience · 2019Article
- Protocol measuring horizontal gene transfer from algae to non-photosynthetic organisms.MethodsX · 2019Article
- Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications.Frontiers in microbiology · 2019Review
- Carrying Capacity and Colonization Dynamics ofFrontiers in microbiology · 2018Article
- Evolution of Chaperonin Gene Duplication in Stigonematalean Cyanobacteria (Subsection V).Genome biology and evolution · 2017Article
- Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications.Frontiers in microbiology · 2016Review
- Establishment of the forward genetic analysis of the chlorophyll d-dominated cyanobacterium Acaryochloris marina MBIC 11017 by applying in vivo transposon mutagenesis system.Photosynthesis research · 2015Article
- Plasmid conjugation from proteobacteria as evidence for the origin of xenologous genes in cyanobacteria.Journal of bacteriology · 2014Article
- Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria.Nucleic acids research · 2014Article
- Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.Nucleic acids research · 2012Article
- Transformation and conjugal transfer of foreign genes into the filamentous multicellular cyanobacteria (subsection V) Fischerella and Chlorogloeopsis.Current microbiology · 2012Article
- Mobility of plasmids.Microbiology and molecular biology reviews : MMBR · 2010Review
- Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology.Nucleic acids research · 2010Article
- Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367.Molecular microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prochlorococcus is the smallest oxygenic phototroph yet described. It numerically dominates the phytoplankton community in the mid-latitude oceanic gyres, where it has an important role in the global carbon cycle. The complete genomes of several Prochlorococcus strains have been sequenced, revealing that nearly half of the genes in each genome are of unknown function. Genetic methods, such as reporter gene assays and tagged mutagenesis, are critical to unveiling the functions of these genes. Here, we describe conditions for the transfer of plasmid DNA into Prochlorococcus strain MIT9313 by interspecific conjugation with Escherichia coli. Following conjugation, E. coli bacteria were removed from the Prochlorococcus cultures by infection with E. coli phage T7. We applied these methods to show that an RSF1010-derived plasmid will replicate in Prochlorococcus strain MIT9313. When this plasmid was modified to contain green fluorescent protein, we detected its expression in Prochlorococcus by Western blotting and cellular fluorescence. Further, we applied these conjugation methods to show that a mini-Tn5 transposon will transpose in vivo in Prochlorococcus. These genetic advances provide a basis for future genetic studies with Prochlorococcus, a microbe of ecological importance in the world's oceans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.