ArticleBiophysical journal2007
Metastable mesoscopic clusters in solutions of sickle-cell hemoglobin.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 118 citations in OpenAlex.
- Navigating Classical and Nonclassical Realms of Nucleation and Crystallization.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Classical and Nonclassical Nucleation Mechanisms of Biomolecules.Advances in biochemical engineering/biotechnology · 2026Review
- A dual growth mode unique for organic crystals relies on mesoscopic liquid precursors.Communications chemistry · 2024Article
- Testing mixing rules for structural and dynamical quantities in multi-component crowded protein solutions.APL bioengineering · 2024Article
- Protein nanocondensates: the next frontier.Biophysical reviews · 2023Review
- NMR and dynamic light scattering give different diffusion information for short-living protein oligomers. Human serum albumin in water solutions of metal ions.European biophysics journal : EBJ · 2022Article
- Nucleation of glucose isomerase protein crystals in a nonclassical disguise: The role of crystalline precursors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Protein conformation and biomolecular condensates.Current research in structural biology · 2022Review
- Interaction of spindle assembly factor TPX2 with importins-α/β inhibits protein phase separation.The Journal of biological chemistry · 2021Article
- Multivalent ions and biomolecules: Attempting a comprehensive perspective.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Review
- The metastable states of proteins.Protein science : a publication of the Protein Society · 2020Review
- Experimental Evidence for a Cluster Glass Transition in Concentrated Lysozyme Solutions.The journal of physical chemistry. B · 2019Article
- Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils.iScience · 2019Article
- Molecular nucleation mechanisms and control strategies for crystal polymorph selection.Nature · 2018Article
- Repetitive Biomimetic Self-healing of Ca(2+)-Induced Nanocomposite Protein Hydrogels.Scientific reports · 2016Article
- Lack of Dependence of the Sizes of the Mesoscopic Protein Clusters on Electrostatics.Biophysical journal · 2015Article
- Staying Together: Protein Molecules in Mesoscopic Clusters.Biophysical journal · 2015Article
- Do protein crystals nucleate within dense liquid clusters?Acta crystallographica. Section F, Structural biology communications · 2015Article
- Experimental and imaging techniques for examining fibrin clot structures in normal and diseased states.Journal of visualized experiments : JoVE · 2015Article
- Nucleation precursors in protein crystallization.Acta crystallographica. Section F, Structural biology communications · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Sickle cell hemoglobin (HbS) is a mutant, whose polymerization while in deoxy state in the venous circulation underlies the debilitating sickle cell anemia. It has been suggested that the nucleation of the HbS polymers occurs within clusters of dense liquid, existing in HbS solutions. We use dynamic light scattering with solutions of deoxy-HbS, and, for comparison, of oxy-HbS and oxy-normal adult hemoglobin, HbA. We show that solutions of all three Hb variants contain clusters of dense liquid, several hundred nanometers in size, which are metastable with respect to the Hb solutions. The clusters form within a few seconds after solution preparation and their sizes and numbers remain relatively steady for up to 3 h. The lower bound of the cluster lifetime is 15 ms. The clusters exist in broad temperature and Hb concentration ranges, and occupy 10(-5)-10(-2) of the solution volume. The results on the cluster properties can serve as test data for a potential future microscopic theory of cluster stability and kinetics. More importantly, if the clusters are a part of the nucleation mechanism of HbS polymers, the rate of HbS polymerization can be controlled by varying the cluster properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.