ArticleBiophysical journal2007
Membrane order and molecular dynamics associated with IgE receptor cross-linking in mast cells.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 64 citations in OpenAlex.
- Critical roles of plasma membrane dynamics and heterogeneity at the early stage of antigen-stimulated FcεRI signaling in mast cells.Biochemical Society transactions · 2025Review
- Antigen properties shape organization of FcεRI aggregates to tune mast cell signaling.bioRxiv : the preprint server for biology · 2025Article
- Synergy between membrane topography and domains to control signaling protein localization in mast cells facilitates their activation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Lipid-based and protein-based interactions synergize transmembrane signaling stimulated by antigen clustering of IgE receptors.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Plasma membrane order and fluidity are diversely triggered by elicitors of plant defence.Journal of experimental botany · 2016Article
- Fluorescence anisotropy (polarization): from drug screening to precision medicine.Expert opinion on drug discovery · 2015Review
- Defining a polymethine dye for fluorescence anisotropy applications in the near-infrared spectral range.Chemphyschem : a European journal of chemical physics and physical chemistry · 2012Article
- A mechanistic model of early FcεRI signaling: lipid rafts and the question of protection from dephosphorylation.PloS one · 2012Article
- Immunoglobulin E receptor signaling and asthma.The Journal of biological chemistry · 2011Review
- Endothelial Cell Membrane Sensitivity to Shear Stress is Lipid Domain Dependent.Cellular and molecular bioengineering · 2011Article
- Lipid rafts in mast cell biology.Journal of lipids · 2011Article
- Fluorescence lifetime measurements and biological imaging.Chemical reviews · 2010Review
- Fluorescence polarization/anisotropy in diagnostics and imaging.Chemical reviews · 2010Review
- Formation of a mast cell synapse: Fc epsilon RI membrane dynamics upon binding mobile or immobilized ligands on surfaces.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Adapters in the organization of mast cell signaling.Immunological reviews · 2009Review
- Time-of-flight secondary ion mass spectrometry imaging of subcellular lipid heterogeneity: Poisson counting and spatial resolution.Analytical chemistry · 2009Article
- Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative.Biophysical journal · 2009Article
- Probing membrane order and topography in supported lipid bilayers by combined polarized total internal reflection fluorescence-atomic force microscopy.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholesterol-rich microdomains (or "lipid rafts") within the plasma membrane have been hypothesized to exist in a liquid-ordered phase and play functionally important roles in cell signaling; however, these microdomains defy detection using conventional imaging. To visualize domains and relate their nanostructure and dynamics to mast cell signaling, we use two-photon (760 nm and 960 nm) fluorescence lifetime imaging microscopy and fluorescence polarization anisotropy imaging, with comparative one-photon anisotropy imaging and single-point lifetime and anisotropy decay measurements. The inherent sensitivity of ultrafast excited-state dynamics and rotational diffusion to the immediate surroundings of a fluorophore allows for real-time monitoring of membrane structure and organization. When the high affinity receptor for IgE (FcepsilonRI) is extensively cross-linked with anti-IgE, molecules associated with cholesterol-rich microdomains (e.g., saturated lipids (the lipid analog diI-C(18) or glycosphingolipids)) and lipid-anchored proteins coredistribute with cross-linked IgE-FcepsilonRI. We find an enhancement in fluorescence lifetime and anisotropy of diI-C(18) and Alexa 488-labeled IgE-FcepsilonRI in the domains where these molecules colocalize. Our results suggest that fluorescence lifetime and, particularly, anisotropy permit us to correlate the recruitment of lipid molecules into more ordered domains that serve as platforms for IgE-mediated signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.