ArticleNucleic acids research2006
The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 112 citations in OpenAlex.
- Posttranscriptional 3'-Terminal Modifications ofMicroorganisms · 2025Article
- Genome-scale CRISPRi screen identifies pcnB repression conferring improved physiology for overproduction of free fatty acids in Escherichia coli.Nature communications · 2025Article
- Deletion of pcnB affects antibiotic susceptibility in resistant Escherichia coli by reducing copy number of ColE1-family plasmids.Scientific reports · 2025Article
- Article
- The emergence and evolution of gene expression in genome regions replete with regulatory motifs.eLife · 2024Article
- Base editor-mediated large-scale screening of functional mutations in bacteria for industrial phenotypes.Science China. Life sciences · 2024Article
- Genome-wide identification of fitness-genes in aminoglycoside-resistant Escherichia coli during antibiotic stress.Scientific reports · 2024Article
- Ubiquitous mRNA decay fragments in E. coli redefine the functional transcriptome.Nucleic acids research · 2022Article
- Regulation of mRNA decay inCritical reviews in biochemistry and molecular biology · 2022Article
- Inactivation of RNase P in Escherichia coli significantly changes post-transcriptional RNA metabolism.Molecular microbiology · 2022Article
- Antiviral Resistance and Phage Counter Adaptation to Antibiotic-Resistant Extraintestinal PathogenicmBio · 2021Article
- Transcriptome-Wide Analysis of Stationary Phase Small ncRNAs inInternational journal of molecular sciences · 2021Article
- Mapping and editing of nucleic acid modifications.Computational and structural biotechnology journal · 2020Review
- Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.Frontiers in microbiology · 2020Review
- New Insights into the Relationship between tRNA Processing and Polyadenylation in Escherichia coli.Trends in genetics : TIG · 2019Review
- Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent.Microbial cell factories · 2018Article
- Poly(A) polymerase is required for RyhB sRNA stability and function inRNA (New York, N.Y.) · 2018Article
- Enzymes Involved in Posttranscriptional RNA Metabolism in Gram-Negative Bacteria.Microbiology spectrum · 2018Review
- Trees, fungi and bacteria: tripartite metatranscriptomics of a root microbiome responding to soil contamination.Microbiome · 2018Article
- Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli.BMC microbiology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Polyadenylation of RNAs by poly(A) polymerase I (PAP I) in Escherichia coli plays a significant role in mRNA decay and general RNA quality control. However, many important features of this system, including the prevalence of polyadenylated mRNAs in the bacterium, are still poorly understood. By comparing the transcriptomes of wild-type and pcnB deletion strains using macroarray analysis, we demonstrate that >90% of E.coli open reading frames (ORFs) transcribed during exponential growth undergo some degree of polyadenylation by PAP I, either as full-length transcripts or decay intermediates. Detailed analysis of over 240 transcripts suggests that Rho-independent transcription terminators serve as polyadenylation signals. Conversely, mRNAs terminated in a Rho-dependent fashion are probably not substrates for PAP I, but can be modified by the addition of long polynucleotide tails through the biosynthetic activity of polynucleotide phosphorylase (PNPase). Furthermore, real-time PCR analysis indicates that the extent of polyadenylation of individual full-length transcripts such as lpp and ompA varies significantly in wild-type cells. The data presented here demonstrates that polyadenylation in E.coli occurs much more frequently than previously envisioned.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.