ArticlePlanta2007
Secretion marker proteins and cell-wall polysaccharides move through different secretory pathways.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
- Colocalising proteins and polysaccharides in plants for cell wall and trafficking studies.Frontiers in plant science · 2024Article
- Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis.Frontiers in plant science · 2023Article
- Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry.Biosensors · 2022Article
- Expression of ExogenousBiology · 2022Article
- Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER.Membranes · 2021Article
- Bundling up the Role of the Actin Cytoskeleton in Primary Root Growth.Frontiers in plant science · 2021Review
- AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana.Journal of integrative plant biology · 2020Article
- Exocyst mutants suppress pollen tube growth and cell wall structural defects of hydroxyproline O-arabinosyltransferase mutants.The Plant journal : for cell and molecular biology · 2020Article
- Auxin-induced actin cytoskeleton rearrangements require AUX1.The New phytologist · 2020Article
- SNARE Complexity in Arbuscular Mycorrhizal Symbiosis.Frontiers in plant science · 2020Article
- CesA6 and PGIP2 Endocytosis Involves Different Subpopulations of TGN-Related Endosomes.Frontiers in plant science · 2020Article
- Post-Golgi Trafficking and Transport of Cell Wall Components.Frontiers in plant science · 2018Review
- The Actin Cytoskeleton: Functional Arrays for Cytoplasmic Organization and Cell Shape Control.Plant physiology · 2018Review
- Annexins as Overlooked Regulators of Membrane Trafficking in Plant Cells.International journal of molecular sciences · 2017Review
- Journey to the cell surface--the central role of the trans-Golgi network in plants.Protoplasma · 2015Review
- Molecular dissection of Phaseolus vulgaris polygalacturonase-inhibiting protein 2 reveals the presence of hold/release domains affecting protein trafficking toward the cell wall.Frontiers in plant science · 2015Article
- Peroxisomes contribute to reactive oxygen species homeostasis and cell division induction in Arabidopsis protoplasts.Frontiers in plant science · 2015Article
- Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis.The Plant cell · 2014Article
- Cellular localization and biochemical characterization of a chimeric fluorescent protein fusion of Arabidopsis cellulose synthase-like A2 inserted into Golgi membrane.TheScientificWorldJournal · 2014Article
- The endoplasmic reticulum is a reservoir for WAVE/SCAR regulatory complex signaling in the Arabidopsis leaf.Plant physiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The building up of the cell wall is tightly dependent on the functionality of the secretory pathway. Syntaxins as well as other SNARE proteins play important roles during vesicle secretion and fusion. We have compared the secretion of newly synthesised cell-wall polysaccharides to that of secretory marker proteins such as secreted green-fluorescent protein (sec-GFP) and secreted rat preputial beta-glucuronidase (secRGUS) in leaf protoplasts and roots of wild-type and transgenic Nicotiana tabacum plants, overexpressing a syntaxin homologue NtSyr1 (Sp1) and its soluble variant Sp2 that interferes specifically with Sp1 function, affecting post-Golgi transport. In protoplasts transiently transformed with secGFP and Sp1, no variation was observed in the pattern of fluorescence with respect to control; on the contrary, GFP fluorescence accumulate within the cells in protoplasts co-transformed with secGFP and Sp2. Sp2 reduced the percentage of marker protein secretion to 53% as quantified with secRGUS. In protoplasts obtained from leaves of wild-type and transformed tobacco plants expressing Sp1, Sp2 and Sp1 plus Sp2, no remarkable differences in the percentage of newly synthesised polysaccharides incorporated into the regenerating cell walls were observed. The same results were confirmed in roots of whole transformed seedlings. Tests with cytochalasin D (CD) showed a marked decrease in the amount of newly synthesised polysaccharides into the wall and a simultaneous sharp increase in membrane-associated polysaccharides. SecRGUS secretion was also inhibited by CD. The data indicate that marker proteins and matrix polysaccharides, as well as cellulose synthase complexes, are secreted through the involvement of different secretory machineries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.