ArticleBlood2007
Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm.
Article in Blood, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 84 citations in OpenAlex.
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- Microengineered In Vitro Assays for Screening and Sorting Manufactured Therapeutic T Cells.Journal of immunology (Baltimore, Md. : 1950) · 2024Review
- Human BBB-on-a-chip reveals barrier disruption, endothelial inflammation, and T cell migration under neuroinflammatory conditions.Frontiers in molecular neuroscience · 2023Article
- Immune cell behaviour and dynamics in the kidney - insights from in vivo imaging.Nature reviews. Nephrology · 2022Review
- Differential immunological signature at the culprit site distinguishes acute coronary syndrome with intact from acute coronary syndrome with ruptured fibrous cap: results from the prospective translational OPTICO-ACS study.European heart journal · 2020Observational
- T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation.Frontiers in physics · 2019Article
- A silicon nanomembrane platform for the visualization of immune cell trafficking across the human blood-brain barrier under flow.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- CD11cFrontiers in immunology · 2019Article
- Biomimetic post-capillary venule expansions for leukocyte adhesion studies.Scientific reports · 2018Article
- Endothelial Cell Focal Adhesion Regulates Transendothelial Migration and Subendothelial Crawling of T Cells.Frontiers in immunology · 2018Article
- Regulation of genome organization and gene expression by nuclear mechanotransduction.Nature reviews. Molecular cell biology · 2017Review
- T-cell regulation through a basic suppressive mechanism targeting low-density lipoprotein receptor-related protein 1.Immunology · 2017Article
- Migration of bone marrow progenitor cells in the adult brain of rats and rabbits.World journal of stem cells · 2016Review
- Modeling leukocyte trafficking at the human blood-nerve barrier in vitro and in vivo geared towards targeted molecular therapies for peripheral neuroinflammation.Journal of neuroinflammation · 2016Review
- Characterization of T cell repertoire of blood, tumor, and ascites in ovarian cancer patients using next generation sequencing.Oncoimmunology · 2015Article
- Article
- Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects.PloS one · 2014Article
- Detection of bidirectional signaling during integrin activation and neutrophil adhesion.Methods in molecular biology (Clifton, N.J.) · 2014Article
- T lymphocytes orient against the direction of fluid flow during LFA-1-mediated migration.Biophysical journal · 2013Article
- α(M)β(2)-integrin-intercellular adhesion molecule-1 interactions drive the flow-dependent trafficking of Guillain-Barré syndrome patient derived mononuclear leukocytes at the blood-nerve barrier in vitro.Journal of cellular physiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Lymphocyte extravasation requires that emigrating cells process chemoattractant signals, typically mediated by chemokines, encountered on endothelial surface (apical) and subendothelial (basal) compartments. These signals are delivered under conditions of hemodynamic shear, a fundamental feature of all physiologic leukocyte-endothelial interactions. To analyze lymphocyte responsiveness to spatially distributed chemokines and their effects on transendothelial migration (TEM) under hydrodynamic shear, we constructed a transwell-based flow assay. We observed that the inflammatory chemokine CCL5 (RANTES) induces negligible human T-cell migration across inflamed human umbilical vascular endothelial cells (HUVECs) when displayed alone in the subendothelial compartment under static or hemodynamic shear conditions or when combined with apical CXCL12 (SDF-1alpha) under static conditions. However, under shear stress, T cells encountering apically presented CXCL12 were primed to undergo robust LFA-1-dependent TEM toward subendothelial CCL5. Notably, locomotive T cells arriving at endothelial junctions were retained and extended pseudopodia into and through the junctions, thereby increasing sensitivity to subendothelial CCL5. These findings provide the first evidence that lymphocytes integrate, conditional to shear forces, permissive apical chemokine deposits, and integrin engagement signals, resulting in morphologic changes and amplified chemotaxis to an otherwise weak subendothelial chemokine signal.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.