ArticleThe Journal of physiology2007
The electrogenicity of the rat sodium-bicarbonate cotransporter NBCe1 requires interactions among transmembrane segments of the transporter.
Article in The Journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Alternative transcription of sodium/bicarbonate transporter SLC4A7 gene enhanced by single nucleotide polymorphisms.Physiological genomics · 2017Article
- Cation-coupled bicarbonate transporters.Comprehensive Physiology · 2014Review
- Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium-bicarbonate cotransporter NBCn1 (SLC4A7).The Journal of physiology · 2013Article
- The divergence, actions, roles, and relatives of sodium-coupled bicarbonate transporters.Physiological reviews · 2013Review
- HCO(3)(-)-independent conductance with a mutant Na(+)/HCO(3)(-) cotransporter (SLC4A4) in a case of proximal renal tubular acidosis with hypokalaemic paralysis.The Journal of physiology · 2012Article
- Effect of simultaneously replacing putative TM6 and TM12 of human NBCe1-A with those from NBCn1 on surface abundance in Xenopus oocytes.The Journal of membrane biology · 2012Article
- SLC4A transporters.Current topics in membranes · 2012Article
- PSD-95 interacts with NBCn1 and enhances channel-like activity without affecting Na/HCO(3) cotransport.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2012Article
- Role of an extracellular loop in determining the stoichiometry of Na+-HCO₃⁻ cotransporters.The Journal of physiology · 2011Article
- Remarkable commonalities of electrogenic and electroneutral Na+-phosphate cotransporters.The Journal of physiology · 2009Article
- Mutation of Aspartate 555 of the Sodium/Bicarbonate Transporter SLC4A4/NBCe1 Induces Chloride Transport.The Journal of biological chemistry · 2009Article
- Modular structure of sodium-coupled bicarbonate transporters.The Journal of experimental biology · 2009Review
- NBCe1-A Transmembrane Segment 1 Lines the Ion Translocation Pathway.The Journal of biological chemistry · 2009Article
- Functional analysis of a novel missense NBC1 mutation and of other mutations causing proximal renal tubular acidosis.Pflugers Archiv : European journal of physiology · 2008Article
- Acute regulation of mouse AE2 anion exchanger requires isoform-specific amino acid residues from most of the transmembrane domain.The Journal of physiology · 2007Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The electrogenic Na+-HCO3- cotransporter (NBCe1) plays a central role in intracellular pH (pHi) regulation as well as HCO3- secretion by pancreatic ducts and HCO3- reabsorption by renal proximal tubules. To understand the structural requirements for the electrogenicity of NBCe1, we constructed chimeras of NBCe1-A and the electroneutral NBCn1-B, and used two-electrode voltage clamp to measure electrogenic transporter current in Xenopus oocytes exposed to 5% CO2-26 mm HCO3- (pH 7.40). The chimera consisting of NBCe1-A (i.e. NBCe1-A 'background') with the cytoplasmic N-terminal domain (Nt) of NBCn1-B had a reversal potential of -156.3 mV (compared with a membrane potential Vm of -43.1 mV in a HCO3(-)-free solution) and a slope conductance of 3.0 microS (compared with 12.5 microS for NBCe1-A). Also electrogenic were chimeras with an NBCe1-A background but with NBCn1-B contributing the extracellular loop (L) between transmembrane segment (TM) 5 and 6 (-140.9 mV/11.1 microS), the cytoplasmic C-terminal domain (Ct; -123.8 mV/9.7 microS) or Nt + L + Ct (-120.9 mV/3.7 microS). Reciprocal chimeras (with an NBCn1 background but with NBCe1 contributing Nt, L, Ct or Nt + L + Ct) produced no measurable electrogenic transporter currents in the presence of CO2-HCO3-. pHi recovered from an acid load, but without the negative shift of Vm that is characteristic of electrogenic Na+-HCO3- cotransporters. Thus, these chimeras were electroneutral, as were two others consisting of NBCe1(Nt-L)/NBCn1(TM6-Ct) and NBCn1(Nt-L)/NBCe1(TM6-Ct). We propose that the electrogenicity of NBCe1 requires interactions between TM1-5 and TM6-13.
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