ArticleMolecular biology of the cell2006
Bypassing the catalytic activity of SIR2 for SIR protein spreading in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- CAF-1 and Rtt101p function within the replication-coupled chromatin assembly network to promote H4 K16ac, preventing ectopic silencing.PLoS genetics · 2020Article
- S-phase-independent silencing establishment ineLife · 2020Article
- Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 inGenetics · 2019Article
- Elevated dosage of Ulp1 disrupts telomeric silencing in Saccharomyces cerevisiae.Molecular biology reports · 2018Article
- Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium.Molecular biology of the cell · 2017Article
- Histone Deacetylases with Antagonistic Roles in Saccharomyces cerevisiae Heterochromatin Formation.Genetics · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Impacts on Sirtuin Function and Bioavailability of the Dietary Bioactive Compound Dihydrocoumarin.PloS one · 2016Article
- Sumoylation of Sir2 differentially regulates transcriptional silencing in yeast.Nucleic acids research · 2015Article
- Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation.The EMBO journal · 2013Article
- Single-molecule tools elucidate H2A.Z nucleosome composition.Journal of cell science · 2012Article
- The SUMO E3 ligase Siz2 exerts a locus-dependent effect on gene silencing in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- Chromatin affinity-precipitation using a small metabolic molecule: its application to analysis of O-acetyl-ADP-ribose.Cellular and molecular life sciences : CMLS · 2012Article
- A dual role of H4K16 acetylation in the establishment of yeast silent chromatin.The EMBO journal · 2011Article
- Spt10 and Spt21 are required for transcriptional silencing in Saccharomyces cerevisiae.Eukaryotic cell · 2011Article
- Proliferating cell nuclear antigen (PCNA) is required for cell cycle-regulated silent chromatin on replicated and nonreplicated genes.The Journal of biological chemistry · 2010Article
- Regulation of yeast sirtuins by NAD(+) metabolism and calorie restriction.Biochimica et biophysica acta · 2010Review
- Rpd3-dependent boundary formation at telomeres by removal of Sir2 substrate.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Expanded roles of the origin recognition complex in the architecture and function of silenced chromatin in Saccharomyces cerevisiae.Molecular and cellular biology · 2010Article
- Repressive and non-repressive chromatin at native telomeres in Saccharomyces cerevisiae.Epigenetics & chromatin · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sir protein spreading along chromosomes and silencing in Saccharomyces cerevisiae requires the NAD+-dependent histone deacetylase activity of Sir2p. We tested whether this requirement could be bypassed at the HM loci and telomeres in cells containing a stably expressed, but catalytically inactive mutant of Sir2p, sir2-345p, plus histone mutants that mimic the hypoacetylated state normally created by Sir2p. Sir protein spreading was rescued in sir2-345 mutants expressing histones in which key lysine residues in their N-termini had been mutated to arginine. Mating in these mutants was also partially restored upon overexpression of Sir3p. Together, these results indicate that histone hypoacetylation is sufficient for Sir protein spreading in the absence of production of 2'-O-acetyl-ADP ribose by sir2p and Sir2p's enzymatic function for silencing can be bypassed in a subset of cells in a given population. These results also provide genetic evidence for the existence of additional critical substrates of Sir2p for silencing in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.