ArticleJournal of clinical microbiology2006
Use of smear-positive samples to assess the PCR-based genotype MTBDR assay for rapid, direct detection of the Mycobacterium tuberculosis complex as well as its resistance to isoniazid and rifampin.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 3 of them syntheses that pooled it.
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Who cites it
27 citing papers in PubMed, 3 syntheses or guidelines pooled it, 70 citations in OpenAlex.
- Systematic review of allelic exchange experiments aimed at identifying mutations that confer drug resistance in Mycobacterium tuberculosis.The Journal of antimicrobial chemotherapy · 2014Pooled it
- Pooled it
- Direct susceptibility testing for multi drug resistant tuberculosis: a meta-analysis.BMC infectious diseases · 2009Pooled it
- New insight in molecular detection of Mycobacterium tuberculosis.AMB Express · 2024Review
- Early detection of multidrug- and pre-extensively drug-resistant tuberculosis from smear-positive sputum by direct sequencing.BMC infectious diseases · 2017Article
- Interplay between Mutations and Efflux in Drug Resistant Clinical Isolates ofFrontiers in microbiology · 2017Article
- Pulmonary Tuberculosis Diagnosis: Where We Are?Tuberculosis and respiratory diseases · 2016Review
- Molecular Mycobacteriology and Expansion in Disease Diagnosis.Indian journal of clinical biochemistry : IJCB · 2016Review
- Genotypic, Phenotypic and Clinical Validation of GeneXpert in Extra-Pulmonary and Pulmonary Tuberculosis in India.PloS one · 2016Article
- The Race Is On To Shorten the Turnaround Time for Diagnosis of Multidrug-Resistant Tuberculosis.Journal of clinical microbiology · 2015Article
- General and advanced diagnostic tools to detect Mycobacterium tuberculosis and their drug susceptibility: a review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Review
- Article
- Rapid determination of anti-tuberculosis drug resistance from whole-genome sequences.Genome medicine · 2015Article
- Enhancement of antibiotic activity by efflux inhibitors against multidrug resistant Mycobacterium tuberculosis clinical isolates from Brazil.Frontiers in microbiology · 2015Article
- Profiling of rpoB mutations and MICs for rifampin and rifabutin in Mycobacterium tuberculosis.Journal of clinical microbiology · 2014Article
- Potential for use of the Seegene Anyplex MTB/NTM real-time detection assay in a regional reference laboratory.Journal of clinical microbiology · 2014Article
- Molecular approaches for detection of the multi-drug resistant tuberculosis (MDR-TB) in Bangladesh.PloS one · 2014Article
- Impact of introducing the line probe assay on time to treatment initiation of MDR-TB in Delhi, India.PloS one · 2014Article
- Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.Clinical microbiology reviews · 2011Review
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoniazid (INH) and rifampin (RIF) are two of the most important antituberculosis drugs, and resistance to both of these drugs can often result in treatment failure and fatal clinical outcome. Resistance to these two first-line drugs is most often attributed to mutations in the katG, inhA, and rpoB genes. Historically, the identification and testing of the susceptibility of Mycobacterium tuberculosis complex (MTBC) strains takes weeks to complete. Rapid detection of resistance using the PCR-based Genotype MTBDR assay (Hain Lifescience GmbH, Nehren, Germany) has the potential to significantly shorten the turnaround time from specimen receipt to reporting of results of susceptibility testing. Therefore, the aim of the present study was to determine (i) the sensitivity and accuracy of the Genotype MTBDR assay for the detection of MTBC strains and (ii) the ability of the assay to detect the presence of INH and RIF resistance-associated mutations in katG and rpoB from samples taken directly from smear-positive clinical specimens. The results were compared with those obtained with the reference BACTEC 460TB system combined with standard DNA sequencing analysis methods for katG, inhA, and rpoB. A total of 92 drug-resistant and 51 pansusceptible smear-positive specimens were included in the study. The Genotype MTBDR assay accurately and rapidly detected MTBC strains in 94.4% of the 143 specimens and showed a sensitivity of 94.4% for katG and 90.9% for rpoB when used directly on smear-positive specimens. The assay correctly identified INH resistance in 48 (84.2%) of the 57 specimens containing strains with resistance to high levels of INH (0.4 microg/ml) and RIF resistance in 25 (96.2%) of the 26 specimens containing RIF-resistant strains.
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