ArticleJournal of virology2006
Construction and mutagenesis of an artificial bicistronic tick-borne encephalitis virus genome reveals an essential function of the second transmembrane region of protein e in flavivirus assembly.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Role of Capsid Anchor in the Morphogenesis of Zika Virus.Journal of virology · 2018Article
- Article
- An in vitro recombination-based reverse genetic system for rapid mutagenesis of structural genes of the Japanese encephalitis virus.Virologica Sinica · 2015Article
- The stress granule component TIA-1 binds tick-borne encephalitis virus RNA and is recruited to perinuclear sites of viral replication to inhibit viral translation.Journal of virology · 2014Article
- Article
- Three-dimensional architecture of tick-borne encephalitis virus replication sites and trafficking of the replicated RNA.Journal of virology · 2013Article
- Generation and genetic stability of tick-borne encephalitis virus mutants dependent on processing by the foot-and-mouth disease virus 3C protease.The Journal of general virology · 2012Article
- Article
- The unique transmembrane hairpin of flavivirus fusion protein E is essential for membrane fusion.Journal of virology · 2011Article
- The helical domains of the stem region of dengue virus envelope protein are involved in both virus assembly and entry.Journal of virology · 2011Article
- The C-terminal helical domain of dengue virus precursor membrane protein is involved in virus assembly and entry.Virology · 2011Article
- Characterization of the GXXXG motif in the first transmembrane segment of Japanese encephalitis virus precursor membrane (prM) protein.Journal of biomedical science · 2010Article
- The length of and nonhydrophobic residues in the transmembrane domain of dengue virus envelope protein are critical for its retention and assembly in the endoplasmic reticulum.Journal of virology · 2010Article
- A trans-complementing recombination trap demonstrates a low propensity of flaviviruses for intermolecular recombination.Journal of virology · 2010Article
- Fusion loop peptide of the West Nile virus envelope protein is essential for pathogenesis and is recognized by a therapeutic cross-reactive human monoclonal antibody.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Helices alpha2 and alpha3 of West Nile virus capsid protein are dispensable for assembly of infectious virions.Journal of virology · 2009Article
- Functional analysis of potential carboxy-terminal cleavage sites of tick-borne encephalitis virus capsid protein.Journal of virology · 2008Article
- Selection and analysis of mutations in an encephalomyocarditis virus internal ribosome entry site that improve the efficiency of a bicistronic flavivirus construct.Journal of virology · 2007Article
- Identification of Potential Lead Molecules for Zika Envelope Protein from In Silico Perspective.Avicenna journal of medical biotechnologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flaviviruses have a monopartite positive-stranded RNA genome, which serves as the sole mRNA for protein translation. Cap-dependent translation produces a polyprotein precursor that is co- and posttranslationally processed by proteases to yield the final protein products. In this study, using tick-borne encephalitis virus (TBEV), we constructed an artificial bicistronic flavivirus genome (TBEV-bc) in which the capsid protein and the nonstructural proteins were still encoded in the cap cistron but the coding region for the surface proteins prM and E was moved to a separate translation unit under the control of an internal ribosome entry site element inserted into the 3' noncoding region. Mutant TBEV-bc was shown to produce particles that packaged the bicistronic RNA genome and were infectious for BHK-21 cells and mice. Compared to wild-type controls, however, TBEV-bc was less efficient in both RNA replication and infectious particle formation. We took advantage of the separate expression of the E protein in this system to investigate the role in viral assembly of the second transmembrane region of protein E (E-TM2), a second copy of which was retained in the cap cistron to fulfill its other role as an internal signal sequence in the polyprotein. Deletion analysis and replacement of the entire TBEV E-TM2 region with its counterpart from another flavivirus revealed that this element, apart from its role as a signal sequence, is important for virion formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.