ArticleBMC molecular biology2006
N-terminal region of Saccharomyces cerevisiae eRF3 is essential for the functioning of the eRF1/eRF3 complex beyond translation termination.
Article in BMC molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Targeting Translation Termination Machinery with Antisense Oligonucleotides for Diseases Caused by Nonsense Mutations.Nucleic acid therapeutics · 2019Article
- The Pub1 and Upf1 Proteins Act in Concert to Protect Yeast from Toxicity of the [PSI⁺] Prion.International journal of molecular sciences · 2018Article
- A protein polymerization cascade mediates toxicity of non-pathological human huntingtin in yeast.Scientific reports · 2015Article
- Cryptic MHC class I-binding peptides are revealed by aminoglycoside-induced stop codon read-through into the 3' UTR.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- An in vivo control map for the eukaryotic mRNA translation machinery.Molecular systems biology · 2013Article
- Sex, prions, and plasmids in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Amyloid-mediated sequestration of essential proteins contributes to mutant huntingtin toxicity in yeast.PloS one · 2012Article
- A small, glutamine-free domain propagates the [SWI(+)] prion in budding yeast.Molecular and cellular biology · 2011Article
- Suicidal [PSI+] is a lethal yeast prion.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast.BMC molecular biology · 2009Article
- Protein inheritance (prions) based on parallel in-register beta-sheet amyloid structures.BioEssays : news and reviews in molecular, cellular and developmental biology · 2008Review
- Appearance and propagation of polyglutamine-based amyloids in yeast: tyrosine residues enable polymer fragmentation.The Journal of biological chemistry · 2008Article
- Prions of fungi: inherited structures and biological roles.Nature reviews. Microbiology · 2007Review
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Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTermination of translation in eukaryotes requires two release factors, eRF1, which recognizes all three nonsense codons and facilitates release of the nascent polypeptide chain, and eRF3 stimulating translation termination in a GTP-depended manner. eRF3 from different organisms possess a highly conservative C region (eRF3C), which is responsible for the function in translation termination, and almost always contain the N-terminal extension, which is inessential and vary both in structure and length. In the yeast Saccharomyces cerevisiae the N-terminal region of eRF3 is responsible for conversion of this protein into the aggregated and functionally inactive prion form.
resultsHere, we examined functional importance of the N-terminal region of a non-prion form of yeast eRF3. The screen for mutations which are lethal in combination with the SUP35-C allele encoding eRF3C revealed the sup45 mutations which alter the N-terminal domain of eRF1 and increase nonsense codon readthrough. However, further analysis showed that synthetic lethality was not caused by the increased levels of nonsense codon readthrough. Dominant mutations in SUP35-C were obtained and characterized, which remove its synthetic lethality with the identified sup45 mutations, thus indicating that synthetic lethality was not due to a disruption of interaction with proteins that bind to this eRF3 region.
conclusionThese and other data demonstrate that the N-terminal region of eRF3 is involved both in modulation of the efficiency of translation termination and functioning of the eRF1/eRF3 complex outside of translation termination.
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