ArticleBiophysical journal1990
Estimating kinetic parameters for single channels with simulation. A general method that resolves the missed event problem and accounts for noise.
Article in Biophysical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Celebrating 50 Years of Single-Channel Recording with the Patch Clamp.The Journal of membrane biology · 2025Review
- A deep learning approach to real-time Markov modeling of ion channel gating.Communications chemistry · 2024Article
- 2D-dwell-time analysis with simulations of ion-channel gating using high-performance computing.Biophysical journal · 2023Article
- MOSAIC: A Modular Single-Molecule Analysis Interface for Decoding Multistate Nanopore Data.Analytical chemistry · 2016Article
- Article
- MCMC estimation of Markov models for ion channels.Biophysical journal · 2011Article
- Voltage-dependent inactivation gating at the selectivity filter of the MthK K+ channel.The Journal of general physiology · 2010Article
- Inositol trisphosphate receptor and ion channel models based on single-channel data.Chaos (Woodbury, N.Y.) · 2009Article
- Four-mode gating model of fast inactivation of sodium channel Nav1.2a.Pflugers Archiv : European journal of physiology · 2008Article
- Analysis of kinetics in noisy systems: application to single molecule tethered particle motion.Biophysical journal · 2007Article
- Estimation of ion channel kinetics from fluctuations of macroscopic currents.Biophysical journal · 2007Article
- A role for the S0 transmembrane segment in voltage-dependent gating of BK channels.The Journal of general physiology · 2007Article
- Modeling of single noninactivating Na+ channels: evidence for two open and several fast inactivated states.Biophysical journal · 2006Article
- The power of two-dimensional dwell-time analysis for model discrimination, temporal resolution, multichannel analysis and level detection.The Journal of membrane biology · 2006Article
- Intracellular Mg2+ influences both open and closed times of a native Ca2+-activated BK channel in cultured human renal proximal tubule cells.The Journal of membrane biology · 2005Article
- A subsequent fit of time series and amplitude histogram of patch-clamp records reveals rate constants up to 1 per microsecond.The Journal of membrane biology · 2005Article
- Model-based fitting of single-channel dwell-time distributions.Biophysical journal · 2004Article
- Applying hidden Markov models to the analysis of single ion channel activity.Biophysical journal · 2002Article
- Hidden Markov modeling for single channel kinetics with filtering and correlated noise.Biophysical journal · 2000Article
- A direct optimization approach to hidden Markov modeling for single channel kinetics.Biophysical journal · 2000Article
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Authors and funding
2 authors.
Funding
Abstract
Analysis of currents recorded from single channels is complicated by the limited time resolution (filtering) of the data which can prevent the detection of brief intervals. Although a number of approaches have been used to correct for the undetected intervals (missed events) when identifying kinetic models and estimating parameters, none of them provide a general method which takes into account the true effects of noise and limited time resolution. This paper presents such a method. The approach is to use simulated single-channel currents to incorporate the true effects of filtering and noise on missed events and interval durations. The simulated currents are then analyzed in a manner identical to that used to analyze the experimental currents. An iterative search process using likelihood comparison of two-dimensional dwell-time distributions obtained from the simulated and experimental single-channel currents then allows the most likely rate constants to be determined. The large errors and false solutions that can result from the more typically applied assumptions of no noise and an absolute dead time (idealized filtering) are excluded by the iterative simulation method, and the correlation information contained in the two-dimensional distributions should increase the ability to distinguish among different gating mechanisms. The iterative simulation method is generally applicable to channels which typically open to a single conductance level. For these channels the method places no restrictions on the proposed gating mechanism or the form of the predicted dwell-time distributions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.