ArticleBiological procedures online2006
Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions.
Article in Biological procedures online, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Review
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- Got Glycogen?: Development and Multispecies Validation of the Novel Preserve, Precipitate, Lyse, Precipitate, Purify (PPLPP) Workflow for Environmental DNA Extraction from Longmire's Preserved Water Samples.Journal of biomolecular techniques : JBT · 2020Article
- Small Noncoding RNA CjNC110 Influences Motility, Autoagglutination, AI-2 Localization, Hydrogen Peroxide Sensitivity, and Chicken Colonization in Campylobacter jejuni.Infection and immunity · 2020Article
- Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association.Nucleic acids research · 2019Article
- Article
- Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection.BMC research notes · 2014Article
- Increased concentration of iodide in airway secretions is associated with reduced respiratory syncytial virus disease severity.American journal of respiratory cell and molecular biology · 2014Article
- Analysis of autoinducer-2 quorum sensing in Yersinia pestis.Infection and immunity · 2013Article
- Transcriptome analysis of acyl-homoserine lactone-based quorum sensing regulation in Yersinia pestis [corrected].PloS one · 2013Article
- Ontogeny of the immune response in the ovine lung.Immunological investigations · 2012Article
- Exogenous administration of vascular endothelial growth factor prior to human respiratory syncytial virus a2 infection reduces pulmonary pathology in neonatal lambs and alters epithelial innate immune responses.Experimental lung research · 2011Article
- A study of alternative splicing in the pig.BMC research notes · 2010Article
- Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia.Pediatric pulmonology · 2010Article
- SPUD qPCR assay confirms PREXCEL-Q softwares ability to avoid qPCR inhibition.Current issues in molecular biology · 2010Article
- Human respiratory syncytial virus A2 strain replicates and induces innate immune responses by respiratory epithelia of neonatal lambs.International journal of experimental pathology · 2009Article
- Gene profiling studies in the neonatal ovine lung show enhancing effects of VEGF on the immune response.Developmental and comparative immunology · 2009Article
- Laser Capture Microdissection Revisited as a Tool for Transcriptomic Analysis: Application of an Excel-Based qPCR Preparation Software (PREXCEL-Q).International journal of biomedical science : IJBS · 2009Article
- The 'PREXCEL-Q Method' for qPCR.International journal of biomedical science : IJBS · 2008Article
- Transcriptome changes in Mycoplasma hyopneumoniae during infection.Infection and immunity · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamically-sound, truly LOG-linear qPCR reactions very quickly. The qPCR problems this invention solves are universal to all qPCR reactions, and it performs all necessary qPCR set-up calculations in about 52 seconds (using a pentium 4 processor) for up to seven qPCR targets and seventy-two samples at a time - calculations that commonly take capable investigators days to finish. We have named this custom Excel-based file system "FocusField2-6GallupqPCRSet-upTool-001" (FF2-6-001 qPCR set-up tool), and are in the process of transforming it into professional qPCR set-up software to be made available in 2007. The current prototype is already fully functional.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.