ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2007
Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles.
Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 45 citations in OpenAlex.
- Inorganic coatings for optimized non-viral transfection of stem cells.Scientific reports · 2013Article
- Immobilization of biomolecules on the surface of inorganic nanoparticles for biomedical applications.Science and technology of advanced materials · 2010Review
- Nanoparticles for biomedical imaging.Expert opinion on drug delivery · 2009Review
- Nanoparticles for applications in cellular imaging.Nanoscale research letters · 2007Article
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4 authors at 2 institutions in 1 country.
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Abstract
Calcium phosphate nanoparticles were prepared by precipitation from water and were then functionalized by DNA. These particles are taken up by living cells and function as gene transfer agents, i.e., the DNA is brought into a cell's nucleus and is incorporated there into the cell's genome (transfection). DNA which encodes for enhanced green fluorescent protein leads to green fluorescence of successfully transfected cells. By adding the red-fluorescing marker tetramethylrhodamine isothiocyanate-bovine serum albumin (TRITC-BSA) to the nanoparticles, their pathway into the cell and within the cell could be followed by fluorescence microscopy. A clear correlation between the uptake of nanoparticles and the efficiency of transfection was found. Aggregates of DNA/TRITC-BSA alone were not able to enter the cells, i.e., the inorganic nanoparticles are necessary as a carrier through the cell membrane.
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