ArticlePflugers Archiv : European journal of physiology2007
Evidence that TRPC4 supports the calcium selective I(CRAC)-like current in human gingival keratinocytes.
Article in Pflugers Archiv : European journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Wound Repair and CaCells · 2024Review
- The Molecular Heterogeneity of Store-Operated CaInternational journal of molecular sciences · 2023Review
- Editorial: The role of calcium and calcium binding proteins in cell physiology and disease.Frontiers in physiology · 2023Article
- TRPC1 channel clustering during store-operated CaFrontiers in physiology · 2023Article
- Store-operated calcium channels in skin.Frontiers in physiology · 2022Review
- Role of Store-Operated CaBiomolecules · 2021Review
- Calcium Channels: Noteworthy Regulators and Therapeutic Targets in Dermatological Diseases.Frontiers in pharmacology · 2021Review
- Store-Operated Calcium Channels as Drug Target in Gastroesophageal Cancers.Frontiers in pharmacology · 2021Review
- Tracing selection signatures in the pig genome gives evidence for selective pressures on a unique curly hair phenotype in Mangalitza.Scientific reports · 2020Article
- TRPC Channels in the SOCE Scenario.Cells · 2020Review
- Possible antagonistic effects of the TRPC4 channel blocker ML204 on MThe Journal of veterinary medical science · 2018Article
- Skin Barrier and Calcium.Annals of dermatology · 2018Review
- Pathophysiological Significance of Store-Operated Calcium Entry in Megakaryocyte Function: Opening New Paths for Understanding the Role of Calcium in Thrombopoiesis.International journal of molecular sciences · 2016Review
- TRP channels in skin: from physiological implications to clinical significances.Biophysics (Nagoya-shi, Japan) · 2015Review
- TRP channels in the skin.British journal of pharmacology · 2014Review
- ORAI1 calcium channel orchestrates skin homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Kv7/M-type potassium channels in rat skin keratinocytes.Pflugers Archiv : European journal of physiology · 2013Article
- Role of the calcium-sensing receptor in calcium regulation of epidermal differentiation and function.Best practice & research. Clinical endocrinology & metabolism · 2013Review
- Reduced TRPC channel expression in psoriatic keratinocytes is associated with impaired differentiation and enhanced proliferation.PloS one · 2011Article
- Specific TRPC6 channel activation, a novel approach to stimulate keratinocyte differentiation.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
We previously demonstrated that high external [Ca(2+)] activated two Ca(2+) currents in human gingival keratinocytes (HGKs): an initial small I(CRAC)-like current and a second large nonspecific cation current (Fatherazi S, Belton CM, Cai S, Zarif S, Goodwin PC, Lamont RJ, Izutsu KT; Pflugers Arch 448:93-104, 2004). It was recently shown that TRPC1, a member of the transient receptor potential protein family, is a component of the store-operated calcium entry mechanism in keratinocytes. To further elucidate the molecular identity of these channels, we investigated the expression of TRPC4 in gingival tissue and in cultured keratinocytes, and the effect of knockdown of TRPC4 expression on the Ca(2+) currents and influx. Immunohistochemistry showed TRPC4 was present in gingival epithelium as well as in HGKs cultured in different [Ca(2+)]s. Results from tissue and cultured HGKs demonstrated TRPC4 expression decreased with differentiation. Knockdown of TRPC4 in proliferating HGKs with antisense oligonucleotides significantly reduced the intracellular [Ca(2+)] increase obtained upon exposure to high external [Ca(2+)]. Antisense knockdown of TRPC4 expression was confirmed by reverse transcriptase polymerase chain reaction, Western blot, and immunofluorescence microscopy of transfected HGKs. Immunofluorescence microscopy and patch clamp measurements in Lucifer-yellow-tagged, antisense-treated HGKs showed attenuation of TRPC4 expression levels as well as attenuation of the I(CRAC)-like current in the same cell, whereas the large nonspecific cation current was unchanged but significantly delayed. Cells transfected with a scrambled TRPC4 oligonucleotide showed no change in either the I(CRAC)-like or nonspecific currents. The results indicate that TRPC4 is an important component of the I(CRAC)-like channel in HGKs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.