ArticleEukaryotic cell2006
Function of the Dictyostelium discoideum Atg1 kinase during autophagy and development.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- The Multifaceted Interactions ofCells · 2024Article
- The nonautophagic functions of autophagy-related proteins.Autophagy · 2024Review
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- Autophagy in metabolism and quality control: opposing, complementary or interlinked functions?Autophagy · 2022Article
- The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.Autophagy · 2021Article
- Aberrant Autophagy Impacts Growth and Multicellular Development in aFrontiers in cell and developmental biology · 2021Article
- Calmodulin-mediated events during the life cycle of the amoebozoan Dictyostelium discoideum.Biological reviews of the Cambridge Philosophical Society · 2020Review
- The physiological regulation of macropinocytosis duringJournal of cell science · 2018Article
- Cubic membrane formation supports cell survival of amoeba Chaos under starvation-induced stress.Protoplasma · 2018Article
- Autophagy in Dictyostelium: Mechanisms, regulation and disease in a simple biomedical model.Autophagy · 2017Review
- Ubiquitin-like Atg8 protein is expressed during autophagy and the encystation process in Naegleria gruberi.Parasitology research · 2017Article
- The opposing actions of target of rapamycin and AMP-activated protein kinase in cell growth control.Cold Spring Harbor perspectives in biology · 2015Review
- Dissecting the function of Atg1 complex in Dictyostelium autophagy reveals a connection with the pentose phosphate pathway enzyme transketolase.Open biology · 2015Article
- An evolutionarily significant unicellular strategy in response to starvation in Dictyostelium social amoebae.F1000Research · 2014Article
- Regulation of aggregate size and pattern by adenosine and caffeine in cellular slime molds.BMC developmental biology · 2012Article
- Article
- Autophagy in protists.Autophagy · 2011Review
- Autophagy-dependent rhodopsin degradation prevents retinal degeneration in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- TOR-dependent control of autophagy: biting the hand that feeds.Current opinion in cell biology · 2010Review
- Autophagy in unicellular eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
When starved, the amoebae of Dictyostelium discoideum initiate a developmental process that results in the formation of fruiting bodies in which stalks support balls of spores. The nutrients and energy necessary for development are provided by autophagy. Atg1 is a protein kinase that regulates the induction of autophagy in the budding yeast Saccharomyces cerevisiae. In addition to a conserved kinase domain, Dictyostelium Atg1 has a C-terminal region that has significant homology to the Caenorhabditis elegans and mammalian Atg1 homologues but not to the budding yeast Atg1. We investigated the function of the kinase and conserved C-terminal domains of D. discoideum Atg1 (DdAtg1) and showed that these domains are essential for autophagy and development. Kinase-negative DdAtg1 acts in a dominant-negative fashion, resulting in a mutant phenotype when expressed in the wild-type cells. Green fluorescent protein-tagged kinase-negative DdAtg1 colocalizes with red fluorescent protein (RFP)-tagged DdAtg8, a marker of preautophagosomal structures and autophagosomes. The conserved C-terminal region is essential for localization of kinase-negative DdAtg1 to autophagosomes labeled with RFP-tagged Dictyostelium Atg8. The dominant-negative effect of the kinase-defective mutant also depends on the C-terminal domain. In cells expressing dominant-negative DdAtg1, autophagosomes are formed and accumulate but seem not to be functional. By using a temperature-sensitive DdAtg1, we showed that DdAtg1 is required throughout development; development halts when the cells are shifted to the restrictive temperature, but resumes when cells are returned to the permissive temperature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.