ArticleEukaryotic cell2006
Cooperation between reverse transcriptase and integrase during reverse transcription and formation of the preintegrative complex of Ty1.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- An interchangeable prion-like domain is required for Ty1 retrotransposition.bioRxiv : the preprint server for biology · 2023Article
- A proteomic screen of Ty1 integrase partners identifies the protein kinase CK2 as a regulator of Ty1 retrotransposition.Mobile DNA · 2022Article
- Ty1 integrase is composed of an active N-terminal domain and a large disordered C-terminal module dispensable for its activity in vitro.The Journal of biological chemistry · 2021Article
- Host factors that promote retrotransposon integration are similar in distantly related eukaryotes.PLoS genetics · 2017Article
- Reverse Transcription in the Saccharomyces cerevisiae Long-Terminal Repeat Retrotransposon Ty3.Viruses · 2017Review
- A self-encoded capsid derivative restricts Ty1 retrotransposition in Saccharomyces.Current genetics · 2016Review
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- Exploring Ty1 retrotransposon RNA structure within virus-like particles.Nucleic acids research · 2013Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- P-body components are required for Ty1 retrotransposition during assembly of retrotransposition-competent virus-like particles.Molecular and cellular biology · 2010Article
- Posttranslational interference of Ty1 retrotransposition by antisense RNAs.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Functional analysis of N-terminal residues of ty1 integrase.Journal of virology · 2009Article
- The chromodomain of Tf1 integrase promotes binding to cDNA and mediates target site selection.Journal of virology · 2009Article
- The diversity of retrotransposons and the properties of their reverse transcriptases.Virus research · 2008Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcriptase (RT) and integrase (IN) play a central role in the replication and transposition of retroelements. Increasing evidence suggests that the interaction between these two enzymes is functional and plays an important role in replication. In the yeast Saccharomyces cerevisiae retrotransposon Ty1, the interaction of IN with RT is critical for the formation of an active conformation of RT. We show here that the RT associated with VLPs is active only if it is in close interaction with IN. To probe the IN-RT cis-trans relationship, we have used a complementation assay based on coexpressing two transposons. We show that IN acts in cis to activate RT and that a functional integrase provided in trans is not able to complement replication and transposition defects of IN deletion or IN active-site mutant elements. Our data support a model in which IN not only interacts closely with RT during reverse transcription but also remains associated with RT during the formation of the preintegrative complex.
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