ArticleThe Journal of cell biology2006
Visualization of mRNA translation in living cells.
Article in The Journal of cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
70 citing papers in PubMed, 116 citations in OpenAlex.
- Locally synthesized glycyl aminoacyl-tRNA synthetase is important for local translation in neurons.Life science alliance · 2026Article
- Insights into Small Organic Molecule Self-Luminous Probes: a Flourishing Frontier in Bioimaging and Sensing.Topics in current chemistry (Cham) · 2026Review
- Zika and dengue viruses differentially modulate host mRNA processing factors defining its virulence.NAR molecular medicine · 2025Article
- A Method for Bioluminescence-Based RNA Monitoring Using Split-Luciferase Reconstitution Techniques.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging.Nature chemical biology · 2023Article
- Biochemical and mechanical regulation of actin dynamics.Nature reviews. Molecular cell biology · 2022Review
- Cap-dependent translation initiation monitored in living cells.Nature communications · 2022Article
- Vasa nucleates asymmetric translation along the mitotic spindle during unequal cell divisions.Nature communications · 2022Article
- Article
- RNA-biology ruling cancer progression? Focus on 3'UTRs and splicing.Cancer metastasis reviews · 2020Review
- Quantification of mRNA translation in live cells using single-molecule imaging.Nature protocols · 2020Article
- Loss of β-Cytoplasmic Actin in the Intestinal Epithelium Increases Gut Barrier PermeabilityFrontiers in cell and developmental biology · 2020Article
- Fluorescence Imaging Methods to Investigate Translation in Single Cells.Cold Spring Harbor perspectives in biology · 2019Review
- Review
- Posttranscriptional and Posttranslational Regulation of Actin.Anatomical record (Hoboken, N.J. : 2007) · 2018Review
- The makings of the 'actin code': regulation of actin's biological function at the amino acid and nucleotide level.Journal of cell science · 2018Review
- Adenylyl cyclase A mRNA localized at the back of cells is actively translated in live chemotaxingJournal of cell science · 2018Article
- Rapid and dynamic arginylation of the leading edge β-actin is required for cell migration.Traffic (Copenhagen, Denmark) · 2018Article
- Article
- Sp100 colocalizes with HPV replication foci and restricts the productive stage of the infectious cycle.PLoS pathogens · 2017Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The role of mRNA localization is presumably to effect cell asymmetry by synthesizing proteins in specific cellular compartments. However, protein synthesis has never been directly demonstrated at the sites of mRNA localization. To address this, we developed a live cell method for imaging translation of beta-actin mRNA. Constructs coding for beta-actin, containing tetracysteine motifs, were transfected into C2C12 cells, and sites of nascent polypeptide chains were detected using the biarsenial dyes FlAsH and ReAsH, a technique we call translation site imaging. These sites colocalized with beta-actin mRNA at the leading edge of motile myoblasts, confirming that they were translating. beta-Actin mRNA lacking the sequence (zipcode) that localizes the mRNA to the cell periphery, eliminated the translation there. A pulse-chase experiment on living cells showed that the recently synthesized protein correlated spatially with the sites of its translation. Additionally, localization of beta-actin mRNA and translation activity was enhanced at cell contacts and facilitated the formation of intercellular junctions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.