ArticleInfection and immunity2007
A Mycobacterium marinum mel2 mutant is defective for growth in macrophages that produce reactive oxygen and reactive nitrogen species.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Pathogenesis of Cutaneous Mycobacterial Infections-International journal of molecular sciences · 2025Review
- Exopolysaccharide production by seven basidiomycetous fungi and their antioxidant and immunomodulatory activities againstFrontiers in cellular and infection microbiology · 2025Article
- An esculentin-1 homolog from a dark-spotted frog (Pelophylax nigromaculatus) possesses antibacterial and immunoregulatory properties.BMC veterinary research · 2024Article
- Impact of small RNA RaoN on nitrosative-oxidative stress resistance and virulence of Salmonella enterica serovar Typhimurium.Journal of microbiology (Seoul, Korea) · 2020Article
- Evaluation of in silico designed inhibitors targeting MelF (Rv1936) against Mycobacterium marinum within macrophages.Scientific reports · 2019Article
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- Application of Fluorescent Protein Expressing Strains to Evaluation of Anti-Tuberculosis Therapeutic Efficacy In Vitro and In Vivo.PloS one · 2016Article
- Identification and functional characterization of the novel Edwardsiella tarda effector EseJ.Infection and immunity · 2015Article
- The BER necessities: the repair of DNA damage in human-adapted bacterial pathogens.Nature reviews. Microbiology · 2015Review
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- Strain specific transcriptional response in Mycobacterium tuberculosis infected macrophages.Cell communication and signaling : CCS · 2012Article
- Random inducible controlled expression (RICE) for identification of mycobacterial virulence genes.Tuberculosis (Edinburgh, Scotland) · 2011Article
- The professional phagocyte Dictyostelium discoideum as a model host for bacterial pathogens.Current drug targets · 2011Review
- Horizontal gene transfers with or without cell fusions in all categories of the living matter.Advances in experimental medicine and biology · 2011Review
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- Imaging tuberculosis with endogenous beta-lactamase reporter enzyme fluorescence in live mice.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Mycobacterium tuberculosis interferes with the response to infection by inducing the host EphA2 receptor.The Journal of infectious diseases · 2009Article
- Protection of Mycobacterium tuberculosis from reactive oxygen species conferred by the mel2 locus impacts persistence and dissemination.Infection and immunity · 2009Article
- Use of gene dosage effects for a whole-genome screen to identify Mycobacterium marinum macrophage infection loci.Infection and immunity · 2008Article
- The Mycobacterium marinum mel2 locus displays similarity to bacterial bioluminescence systems and plays a role in defense against reactive oxygen and nitrogen species.BMC microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Macrophages produce reactive oxygen species (ROS) and reactive nitrogen species (RNS) in response to bacterial infections. Mycobacteria are relatively resistant to ROS, but RNS inhibit growth of, and possibly even kill, mycobacteria in activated macrophages. We recently constructed a Mycobacterium marinum mel2 locus mutant, which is known to affect macrophage infection. We found previously that the mel2 locus confers resistance to ROS and RNS in laboratory medium, suggesting that this locus might play a similar role during growth in macrophages. Since J774A.1 murine macrophages produce high levels of ROS and RNS upon activation with gamma interferon (IFN-gamma), we examined the effects of IFN-gamma on ROS and RNS production by these cells as well as the effects on growth of M. marinum in these cells. We found that an M. marinum mutant with mutation of the first gene in the mel2 locus, melF, is defective for growth in IFN-gamma-plus-lipopolysaccharide-treated J774A.1 cells and that this defect is abrogated by the presence of either inhibitors of nitric oxide synthase or ROS scavengers. Furthermore, the M. marinum melF mutant displays a defect at late stages in the mouse footpad model of infection. These phenotypic characteristics could be complemented fully by the entire mel2 locus but only partially by the presence of melF alone, supporting data suggesting that this insertion mutation has polar effects on downstream genes in the mel2 locus. These observations demonstrate that the M. marinum mel2 locus plays a role in resistance to ROS and RNS produced by activated macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.