ArticleThe Biochemical journal2007
Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Exploring the Potential of CSTF1 as a Prognostic Biomarker in Hepatocellular Carcinoma and Its Correlation with Immune Infiltration.Journal of hepatocellular carcinoma · 2025Article
- A Novel Gene Pair CSTF2/DPE2A Impacts Prognosis and Cell Cycle of Hepatocellular Carcinoma.Journal of hepatocellular carcinoma · 2023Article
- The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity.Nucleic acids research · 2018Article
- Cstf2t Regulates expression of histones and histone-like proteins in male germ cells.Andrology · 2018Article
- TauCstF-64 Mediates Correct mRNA Polyadenylation and Splicing of Activator and Repressor Isoforms of the Cyclic AMP-Responsive Element Modulator (CREM) in Mouse Testis.Biology of reproduction · 2016Article
- The Cstf2t Polyadenylation Gene Plays a Sex-Specific Role in Learning Behaviors in Mice.PloS one · 2016Article
- The end of the message: multiple protein-RNA interactions define the mRNA polyadenylation site.Genes & development · 2015Review
- mRNA 3'end processing: A tale of the tail reaches the clinic.EMBO molecular medicine · 2014Review
- Overlapping and distinct functions of CstF64 and CstF64τ in mammalian mRNA 3' processing.RNA (New York, N.Y.) · 2013Article
- Mechanisms and consequences of alternative polyadenylation.Molecular cell · 2011Review
- Interactions of CstF-64, CstF-77, and symplekin: implications on localisation and function.Molecular biology of the cell · 2011Article
- The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.The Journal of biological chemistry · 2010Article
- A family of splice variants of CstF-64 expressed in vertebrate nervous systems.BMC molecular biology · 2009Article
- Pre-mRNA 3'-end processing complex assembly and function.Wiley interdisciplinary reviews. RNAReview
- Signals for pre-mRNA cleavage and polyadenylation.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
CstF-64 (cleavage stimulation factor-64), a major regulatory protein of polyadenylation, is absent during male meiosis. Therefore a paralogous variant, tauCstF-64 is expressed in male germ cells to maintain normal spermatogenesis. Based on sequence differences between tauCstF-64 and CstF-64, and on the high incidence of alternative polyadenylation in testes, we hypothesized that the RBDs (RNA-binding domains) of tauCstF-64 and CstF-64 have different affinities for RNA elements. We quantified K(d) values of CstF-64 and tauCstF-64 RBDs for various ribopolymers using an RNA cross-linking assay. The two RBDs had similar affinities for poly(G)18, poly(A)18 or poly(C)18, with affinity for poly(C)18 being the lowest. However, CstF-64 had a higher affinity for poly(U)18 than tauCstF-64, whereas it had a lower affinity for poly(GU)9. Changing Pro-41 to a serine residue in the CstF-64 RBD did not affect its affinity for poly(U)18, but changes in amino acids downstream of the C-terminal alpha-helical region decreased affinity towards poly(U)18. Thus we show that the two CstF-64 paralogues differ in their affinities for specific RNA sequences, and that the region C-terminal to the RBD is mportant in RNA sequence recognition. This supports the hypothesis that tauCstF-64 promotes germ-cell-specific patterns of polyadenylation by binding to different downstream sequence elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.