ArticleThe Plant cell2006
Loading of Arabidopsis centromeric histone CENH3 occurs mainly during G2 and requires the presence of the histone fold domain.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed, 206 citations in OpenAlex.
- Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026Review
- The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3.Plant communications · 2026Article
- Direct haploid formation in Arabidopsis using transgenic CENH3-based inducers.Plant cell reports · 2025Article
- Article
- Ubiquitin-dependent proteolysis of KNL2 driven by APC/CCDC20 is critical for centromere integrity and mitotic fidelity.The Plant cell · 2025Article
- Perspectives of Genome Editing Mediated Haploid Inducer Systems in Legumes.International journal of molecular sciences · 2025Review
- Microfluidics to Follow Spatiotemporal Dynamics at the Nucleo-Cytoplasmic Interface During Plant Root Growth.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Plant chromosome polytenization contributes to suppression of root growth in high polyploids.Journal of experimental botany · 2024Article
- The Chaperone NASP Contributes to de Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis.Plant & cell physiology · 2024Article
- RNAi-mediated downregulation of AcCENH3 can induce in vivo haploids in onion (Allium cepa L.).Scientific reports · 2024Article
- Article
- The structure, function, and evolution of plant centromeres.Genome research · 2024Review
- In vitro co-expression chromatin assembly and remodeling platform for plant histone variants.Scientific reports · 2024Article
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Decoding allelic diversity, transcript variants and transcriptional complexity of CENH3 gene in Brassica oleracea var. botrytis.Protoplasma · 2023Article
- Repression of CENP-A assembly in metaphase requires HJURP phosphorylation and inhibition by M18BP1.The Journal of cell biology · 2023Article
- Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Article
- Advances in biological functions and mechanisms of histone variants in plants.Frontiers in genetics · 2023Review
- Recurrent Plant-Specific Duplications of KNL2 and Its Conserved Function as a Kinetochore Assembly Factor.Molecular biology and evolution · 2022Article
- Centromere Identity and the Regulation of Chromosome Segregation.Frontiers in cell and developmental biology · 2022Review
44 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The centromeric histone H3 (CENH3) substitutes histone H3 within the nucleosomes of active centromeres in all eukaryotes. CENH3 deposition at centromeres is needed to assemble the kinetochore, a complex of conserved proteins responsible for correct chromosome segregation during nuclear division. Histones of regular nucleosomes are loaded during replication in S phase, while CENH3 deposition deviates from this pattern in yeast, human, and Drosophila melanogaster cells. Little is known about when and how CENH3 targets centromeric loci. Therefore, we determined the location and quantity of recombinant enhanced yellow fluorescent protein (EYFP)-CENH3 in mitotic root and endopolyploid leaf nuclei of transgenic Arabidopsis thaliana cells. Our data indicate significant loading of A. thaliana CENH3 during G2 (before splitting into sister kinetochores) rather than during the S or M phase of the cell cycle. The histone fold domain of the C-terminal part of CENH3 is sufficient to target A. thaliana centromeres. A. thaliana EYFP-CENH3 can recognize and target three different centromeric repeats of Arabidopsis lyrata but not field bean (Vicia faba) centromeres.
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