ArticleBiophysical journal2007
Dynamics of the nucleotide pocket of myosin measured by spin-labeled nucleotides.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Molecular mechanisms underlying deoxy-ADP.Pi activation of pre-powerstroke myosin.Protein science : a publication of the Protein Society · 2017Article
- The myosin inhibitor blebbistatin stabilizes the super-relaxed state in skeletal muscle.Biophysical journal · 2014Article
- The histone H4 tail regulates the conformation of the ATP-binding pocket in the SNF2h chromatin remodeling enzyme.Journal of molecular biology · 2014Article
- Effects of ATP and actin-filament binding on the dynamics of the myosin II S1 domain.Biophysical journal · 2013Article
- Conformational changes at the nucleotide site in the presence of bound ADP do not set the velocity of fast Drosophila myosins.Journal of muscle research and cell motility · 2013Article
- Mn(2+)-nucleotide coordination at the myosin active site as detected by pulsed electron paramagnetic resonance.The journal of physical chemistry. B · 2012Article
- Early stages of the recovery stroke in myosin II studied by molecular dynamics simulations.Protein science : a publication of the Protein Society · 2011Article
- EPR spectra and molecular dynamics agree that the nucleotide pocket of myosin V is closed and that it opens on binding actin.Journal of molecular biology · 2011Article
- Switch I closure simultaneously promotes strong binding to actin and ADP in smooth muscle myosin.The Journal of biological chemistry · 2011Article
- Multiple conformations of the nucleotide site of Kinesin family motors in the triphosphate state.Journal of molecular biology · 2011Article
- Nucleotide pocket thermodynamics measured by EPR reveal how energy partitioning relates myosin speed to efficiency.Journal of molecular biology · 2011Article
- Structural kinetics of myosin by transient time-resolved FRET.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The conserved L5 loop establishes the pre-powerstroke conformation of the Kinesin-5 motor, eg5.Biophysical journal · 2010Article
- Article
- Site-directed spectroscopic probes of actomyosin structural dynamics.Annual review of biophysics · 2009Review
- Actin-binding cleft closure in myosin II probed by site-directed spin labeling and pulsed EPR.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Characterization of the pre-force-generation state in the actomyosin cross-bridge cycle.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We have used electron paramagnetic probes attached to the ribose of ATP (SL-ATP) to monitor conformational changes in the nucleotide pocket of myosin. Spectra for analogs bound to myosin in the absence of actin showed a high degree of immobilization, indicating a closed nucleotide pocket. In the Actin.Myosin.SL-AMPPNP, Actin.Myosin.SL-ADP.BeF(3), and Actin.Myosin.SL-ADP.AlF(4) complexes, which mimic weakly binding states near the beginning of the power stroke, the nucleotide pocket remained closed. The spectra of the strongly bound Actin.Myosin.SL-ADP complex consisted of two components, one similar to the closed pocket and one with increased probe mobility, indicating a more open pocket, The temperature dependence of the spectra showed that the two conformations of the nucleotide pocket were in equilibrium, with the open conformation more favorable at higher temperatures. These results, which show that opening of the pocket occurs only in the strongly bound states, appear reasonable, as this would tend to keep ADP bound until the end of the power stroke. This conclusion also suggests that force is initially generated by a myosin with a closed nucleotide pocket.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.