ArticleBiophysical journal2007
Microtubule protofilament number is modulated in a stepwise fashion by the charge density of an enveloping layer.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The vimentin cytoskeleton: when polymer physics meets cell biology.Physical biology · 2020Article
- Minireview - Microtubules and Tubulin Oligomers: Shape Transitions and Assembly by Intrinsically Disordered Protein Tau and Cationic Biomolecules.Langmuir : the ACS journal of surfaces and colloids · 2019Review
- Article
- On the Origin of Microtubules' High-Pressure Sensitivity.Biophysical journal · 2018Article
- The effect of multivalent cations and Tau on paclitaxel-stabilized microtubule assembly, disassembly, and structure.Advances in colloid and interface science · 2016Review
- Direct force measurements reveal that protein Tau confers short-range attractions and isoform-dependent steric stabilization to microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Transformation of taxol-stabilized microtubules into inverted tubulin tubules triggered by a tubulin conformation switch.Nature materials · 2014Article
- Mechanical properties of doubly stabilized microtubule filaments.Biophysical journal · 2013Article
- Ion specific effects in bundling and depolymerization of taxol-stabilized microtubules.Faraday discussions · 2013Article
- Nanoscale assembly in biological systems: from neuronal cytoskeletal proteins to curvature stabilizing lipids.Advanced materials (Deerfield Beach, Fla.) · 2011Review
- Human microtubule-associated-protein tau regulates the number of protofilaments in microtubules: a synchrotron x-ray scattering study.Biophysical journal · 2009Article
- The multiple faces of self-assembled lipidic systems.PMC biophysics · 2009Article
- Block liposomes from curvature-stabilizing lipids: connected nanotubes, -rods, or -spheres.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Block liposomes vesicles of charged lipids with distinctly shaped nanoscale sphere-, pear-, tube-, or rod-segments.Methods in enzymology · 2009Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Microtubules are able to adjust their protofilament (PF) number and, as a consequence, their dynamics and function, to the assembly conditions and presence of cofactors. However, the principle behind such variations is poorly understood. Using synchrotron x-ray scattering and transmission electron microscopy, we studied how charged membranes, which under certain conditions can envelop pre-assembled MTs, regulate the PF number of those MTs. We show that the mean PF number, <N>, is modulated primarily by the charge density of the membranes. <N> decreases in a stepwise fashion with increasing membrane charge density. <N> does not depend on the membrane-protein stoichiometry or the solution ionic strength. We studied the effect of taxol and found that <N> increases logarithmically with taxol/tubulin stoichiometry. We present a theoretical model, which by balancing the electrostatic and elastic interactions in the system accounts for the trends in our findings and reveals an effective MT bending stiffness of order 10-100 k(B)T/nm, associated with the observed changes in PF number.
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