ArticleThe Journal of cell biology1991
The ultrastructure of patch-clamped membranes: a study using high voltage electron microscopy.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 111 citations in OpenAlex.
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension.Nature communications · 2016Article
- Mechanical sensitivity of Piezo1 ion channels can be tuned by cellular membrane tension.eLife · 2015Article
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- Ethanol Effect on BK Channels is Modulated by Magnesium.Alcoholism, clinical and experimental research · 2015Article
- Sensory mechanotransduction at membrane-matrix interfaces.Pflugers Archiv : European journal of physiology · 2015Review
- Gigaseal mechanics: creep of the gigaseal under the action of pressure, adhesion, and voltage.The journal of physical chemistry. B · 2014Article
- Gd3+ and calcium sensitive, sodium leak currents are features of weak membrane-glass seals in patch clamp recordings.PloS one · 2014Article
- Surface properties of glass micropipettes and their effect on biological studies.Nanoscale research letters · 2011Article
- Voltage-gated proton channels: what's next?The Journal of physiology · 2008Review
- Ionic requirements for membrane-glass adhesion and giga seal formation in patch-clamp recording.Biophysical journal · 2007Article
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
- Dopamine transporters depolarize neurons by a channel mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- All-trans-retinal is a closed-state inhibitor of rod cyclic nucleotide-gated ion channels.The Journal of general physiology · 2004Article
- Gadolinium effects on gigaseal formation and the adhesive properties of a fungal amoeboid cell, the slime mutant of Neurospora crassa.The Journal of membrane biology · 2004Article
- Cloning and characterization of glioma BK, a novel BK channel isoform highly expressed in human glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes.The Journal of physiology · 2000Article
- Voltage-induced membrane displacement in patch pipettes activates mechanosensitive channels.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Practical limits on the maximal speed of solution exchange for patch clamp experiments.Biophysical journal · 1999Article
- Disruption of mitochondrial respiration inhibits volume-regulated anion channels and provokes neuronal cell swelling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have developed techniques for studying patch-clamped membranes inside glass pipettes using high voltage electron microscopy (HVEM). To preserve the patch structure with the least possible distortion, we rapidly froze and freeze dried the pipette tip. The pipette is transparent for more than 50 microns from the tip. HVEM images of patches confirm light microscopy observations that the patch is not a bare bilayer, but a membrane-covered bleb of cytoplasm that may include organelles and cytoskeleton. The membrane that spans the pipette is commonly tens of micrometers from the tip of the pipette and occasionally as far as 100 microns. The structure of patches taken from a single cell type is variable but there are consistent differences between patches made from different cell types. With suction applied to the pipette before seal formation, we have seen in the light microscope vesicles swept from the plasmalemma up the pipette. These vesicles are visible in electron micrographs, particularly those made from chick cardiac muscle. Colloidal gold labeling of the patch permitted identification of lectin-binding sites and acetylcholine receptors. In young cultures of Xenopus myocytes, the receptors were diffuse. In 1-wk-old cultures, the receptors formed densely packed arrays. The patch pipette can serve, not only as a recording device, but as a tool for sampling discrete regions of the cell surface. Because the pipette has a constant path length for axial rotation, it is a unique specimen holder for microtomography. We have made preliminary tomographic reconstructions of a patch from Xenopus oocyte.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.