ArticleThe EMBO journal2006
Dynein-mediated pulling forces drive rapid mitotic spindle elongation in Ustilago maydis.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- Mechanisms of nuclear segregation in a multinucleate multibudding yeast.The Journal of cell biology · 2025Article
- Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.eLife · 2021Article
- Electron tomography reveals aspects of spindle structure important for mechanical stability at metaphase.Molecular biology of the cell · 2020Article
- Article
- Cryo-EM structure of the Ustilago maydis kinesin-5 motor domain bound to microtubules.Journal of structural biology · 2019Article
- Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans.PLoS genetics · 2019Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Article
- Increased lateral microtubule contact at the cell cortex is sufficient to drive mammalian spindle elongation.Molecular biology of the cell · 2017Article
- Mechanism of nuclear movements in a multinucleated cell.Molecular biology of the cell · 2017Article
- Anaphase B.Biology · 2016Review
- Understanding force-generating microtubule systems through in vitro reconstitution.Cell adhesion & migration · 2016Review
- Mitosis.Cold Spring Harbor perspectives in biology · 2016Review
- A comprehensive model to predict mitotic division in budding yeasts.Molecular biology of the cell · 2015Article
- Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Function and regulation of dynein in mitotic chromosome segregation.Chromosoma · 2014Review
- Cell cycle-regulated membrane binding of NuMA contributes to efficient anaphase chromosome separation.Molecular biology of the cell · 2014Article
- NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function.The EMBO journal · 2013Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spindle elongation segregates chromosomes and occurs in anaphase, an essential step in mitosis. Dynein-mediated pulling forces position the spindle, but their role in anaphase is a matter of debate. Here, we demonstrate that dynein is responsible for rapid spindle elongation in the model fungus Ustilago maydis. We show that initial slow elongation is supported by kinesin-5, which is located in the spindle mid-zone. When the spindle reaches approximately 2 microm in length, the elongation rate increases four-fold. This coincides with the appearance of long and less-dynamic microtubules (MTs) at each pole that accumulate dynein at their tips. Laser-mediated nanosurgery revealed that these MTs exert pulling forces in control cells, but not in dynein mutants. In addition, dynein mutants undergo initial slow anaphase, but fail to establish less-dynamic MTs and do not perform rapid spindle elongation, suggesting that dynein drives anaphase B. This is most likely mediated by cortical sliding of astral MTs along stationary dynein, which is off-loaded from the MT plus-end to the cortex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.